LOCALIZATION AND ORGANIZATION OF PHENOL DEGRADATION GENES OF PSEUDOMONAS-PUTIDA STRAIN-H

被引：72

作者：

HERRMANN, H

MULLER, C

SCHMIDT, I

MAHNKE, J

PETRUSCHKA, L

HAHNKE, K

机构：

[1] Institut für Genetik und Biochemie der Fachrichtung Biologie, Ernst-Moritz-Arndt-Universität Greifswald, Greifswald

来源：

MOLECULAR & GENERAL GENETICS | 1995年 / 247卷 / 02期

关键词：

PSEUDOMONAS PUTIDA; PHENOL DEGRADATION; INSERTION MUTAGENESIS; GENE ORGANIZATION; DNA SEQUENCE;

D O I：

10.1007/BF00705655

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The genetic organization of the DNA region encoding the phenol degradation pathway of Pseudomonas putida H has been investigated. This strain can utilize phenol or some of its methylated derivatives as its sole source of carbon and energy. The first step in this process is the conversion of phenol into catechol. Catechol is then further metabolized via the meta-cleavage pathway into TCA cycle intermediates. Genes encoding these enzymes are clustered on the plasmid pPGH1. A region of contiguous DNA spanning about 16 kb contains all of the genetic information necessary for inducible phenol degradation. The analysis of mutants generated by insertion of transposons and cassettes indicates that all of the catabolic genes are contained in a single operon. This codes for a multicomponent phenol hydroxylase and meta-cleavage pathway enzymes. Catabolic genes are subject to positive control by the gene product(s) of a second locus.

引用

页码：240 / 246

页数：7

共 38 条

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