NEUROTROPHIN-3;
BRAIN-DERIVED NEUROTROPHIC FACTOR;
ORGAN OF CORTI;
VESTIBULAR ORGANS;
COCHLEOVESTIBULAR NEURONS;
HYBRIDIZATION;
INSITU;
D O I:
10.1016/0378-5955(93)90202-C
中图分类号:
R36 [病理学];
R76 [耳鼻咽喉科学];
学科分类号:
100104 ;
100213 ;
摘要:
In situ hybridization was used to study the expression of mRNAs of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), neurotrophin-5 (NT-5) and the components of their high-affinity receptors in the early postnatal and adult rat inner ears. NGF or NT-5 transcripts were not detected in the inner ear neuroepithelium or in the innervating neurons. NT-3 mRNA was intensely expressed over the one-week-old and adult inner hair cells (IHCs) but in the outer hair cells (OHCs) and vestibular maculae only during the early postnatal period. BDNF mRNA was expressed in the IHCs and OHCs of the early postnatal cochlea but not in the adult organ of Corti. High levels of BDNF transcripts were observed in the sensory epithelia of all vestibular end organs. mRNAs of low affinity NGF receptor. trk B and trk C, but not of trk, were expressed in the spiral and vestibular ganglia. In addition, the non-catalytic form of trk B mRNA localized to the sensory epithelia of maculae utriculi and sacculi. The present results show that of the neurotrophins examined, NT-3 is the predominant neurotrophin in the adult organ of Corti and BDNF is that in vestibular organs. The expression patterns of NT-3 and BDNF mRNAs suggest that these neurotrophins may participate in the maintenance of mature cochleovestibular neurons and they may be involved in the survival response of injured neurons.