EXPRESSION PATTERNS OF NEUROTROPHIN AND THEIR RECEPTOR MESSENGER-RNAS IN THE RAT INNER-EAR

被引:263
作者
YLIKOSKI, J
PIRVOLA, U
MOSHNYAKOV, M
PALGI, J
ARUMAE, U
SAARMA, M
机构
[1] UNIV HELSINKI,DEPT PATHOL,SF-00100 HELSINKI 10,FINLAND
[2] UNIV HELSINKI,INST BIOTECHNOL,SF-00100 HELSINKI 10,FINLAND
[3] ESTONIAN ACAD SCI,INST CHEM PHYS & BIOPHYS,TALLINN,ESTONIA
关键词
NEUROTROPHIN-3; BRAIN-DERIVED NEUROTROPHIC FACTOR; ORGAN OF CORTI; VESTIBULAR ORGANS; COCHLEOVESTIBULAR NEURONS; HYBRIDIZATION; INSITU;
D O I
10.1016/0378-5955(93)90202-C
中图分类号
R36 [病理学]; R76 [耳鼻咽喉科学];
学科分类号
100104 ; 100213 ;
摘要
In situ hybridization was used to study the expression of mRNAs of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), neurotrophin-5 (NT-5) and the components of their high-affinity receptors in the early postnatal and adult rat inner ears. NGF or NT-5 transcripts were not detected in the inner ear neuroepithelium or in the innervating neurons. NT-3 mRNA was intensely expressed over the one-week-old and adult inner hair cells (IHCs) but in the outer hair cells (OHCs) and vestibular maculae only during the early postnatal period. BDNF mRNA was expressed in the IHCs and OHCs of the early postnatal cochlea but not in the adult organ of Corti. High levels of BDNF transcripts were observed in the sensory epithelia of all vestibular end organs. mRNAs of low affinity NGF receptor. trk B and trk C, but not of trk, were expressed in the spiral and vestibular ganglia. In addition, the non-catalytic form of trk B mRNA localized to the sensory epithelia of maculae utriculi and sacculi. The present results show that of the neurotrophins examined, NT-3 is the predominant neurotrophin in the adult organ of Corti and BDNF is that in vestibular organs. The expression patterns of NT-3 and BDNF mRNAs suggest that these neurotrophins may participate in the maintenance of mature cochleovestibular neurons and they may be involved in the survival response of injured neurons.
引用
收藏
页码:69 / 78
页数:10
相关论文
共 65 条
[1]   PLASTICITY OF SUBSTANCE-P IN MATURE AND AGED SYMPATHETIC NEURONS IN CULTURE [J].
ADLER, JE ;
BLACK, IB .
SCIENCE, 1984, 225 (4669) :1499-1500
[2]   TROPHIC INTERACTIONS BETWEEN THE COCHLEO-VESTIBULAR GANGLION OF THE CHICK-EMBRYO AND ITS SYNAPTIC TARGETS IN CULTURE [J].
ARD, MD ;
MOREST, DK ;
HAUGER, SH .
NEUROSCIENCE, 1985, 16 (01) :151-170
[3]   BINDING OF NERVE GROWTH-FACTOR RECEPTOR IN SYMPATHETIC-GANGLIA [J].
BANERJEE, SP ;
SNYDER, SH ;
CUATRECASAS, P ;
GREENE, LA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (09) :2519-2523
[4]   PURIFICATION OF A NEW NEUROTROPHIC FACTOR FROM MAMMALIAN BRAIN [J].
BARDE, YA ;
EDGAR, D ;
THOENEN, H .
EMBO JOURNAL, 1982, 1 (05) :549-553
[5]  
BAYER SA, 1982, SCIENCE, V216, P890, DOI 10.1126/science.7079742
[6]   NEUROTROPHIN-5 - A NOVEL NEUROTROPHIC FACTOR THAT ACTIVATES TRK AND TRKB [J].
BERKEMEIER, LR ;
WINSLOW, JW ;
KAPLAN, DR ;
NIKOLICS, K ;
GOEDDEL, DV ;
ROSENTHAL, A .
NEURON, 1991, 7 (05) :857-866
[7]   MOLECULAR MOTORS IN THE NERVOUS-SYSTEM [J].
BRADY, ST .
NEURON, 1991, 7 (04) :521-533
[8]   TIMING AND SITE OF NERVE GROWTH-FACTOR SYNTHESIS IN DEVELOPING SKIN IN RELATION TO INNERVATION AND EXPRESSION OF THE RECEPTOR [J].
DAVIES, AM ;
BANDTLOW, C ;
HEUMANN, R ;
KORSCHING, S ;
ROHRER, H ;
THOENEN, H .
NATURE, 1987, 326 (6111) :353-358
[9]   EVIDENCE THAT ENDOGENOUS BETA-NERVE GROWTH-FACTOR IS RESPONSIBLE FOR THE COLLATERAL SPROUTING, BUT NOT THE REGENERATION, OF NOCICEPTIVE AXONS IN ADULT-RATS [J].
DIAMOND, J ;
COUGHLIN, M ;
MACINTYRE, L ;
HOLMES, M ;
VISHEAU, B .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (18) :6596-6600
[10]   VESTIBULAR GANGLION OF THE SQUIRREL-MONKEY [J].
FERMIN, CD ;
IGARASHI, M .
ANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY, 1982, 91 (01) :44-52