HPLC RESOLUTION OF HYDROXYL CARBOXYLIC-ACID ENANTIOMERS USING 2-QUINOXALOYL CHLORIDE AS A NEW PRECOLUMN DERIVATIZING AGENT

被引：13

作者：

BRIGHTWELL, M

PAWLOWSKA, M

ZUKOWSKI, J

机构：

[1] SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, Third Avenue, Harlow, Essex

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1995年 / 18卷 / 14期

关键词：

D O I：

10.1080/10826079508009323

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Chiral alpha-hydroxy carboxylic acids were reacted using 2-quinoxaloyl chloride to form UV and fluorescent derivatives. Under mild conditions either in aqueous or non-aqueous conditions reaction proceeds quickly and without racemisation. The labelled compounds have been resolved into enantiomers on beta-cyclodextrin bonded stationary phase operated with polar organic eluents. The high sensitivity and selectivity of the method has been used for the determination of low levels of enantiomeric impurities in commercial ''pure'' samples.

引用

收藏

页码：2765 / 2781

页数：17

相关论文

共 14 条

[1] FACTORS CONTROLLING THE LEVEL AND DETERMINATION OF D-AMINO ACIDS IN THE URINE AND PLASMA OF LABORATORY RODENTS [J].

ARMSTRONG, DW ;

GASPER, MP ;

LEE, SH ;

ERCAL, N ;

ZUKOWSKI, J .

AMINO ACIDS, 1993, 5 (02) :299-315

[2] STEREOCHEMISTRY OF PIPECOLIC ACID FOUND IN THE URINE AND PLASMA OF SUBJECTS WITH PEROXISOMAL DEFICIENCIES [J].

ARMSTRONG, DW ;

ZUKOWSKI, J ;

ERCAL, N ;

GASPER, M .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1993, 11 (10) :881-886

[3] RESOLUTION OF UNDERIVATIZED 2-HYDROXY ACIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

BENECKE, I .

JOURNAL OF CHROMATOGRAPHY, 1984, 291 (MAY) :155-164

[4] THE DETECTION OF ABNORMAL METABOLITES IN MCAD DEFICIENCY - A NEW METHOD [J].

BLOM, W ;

POLDERMOL, AC ;

KELHOLTDIJKMAN, HH ;

HIERCK, L ;

HUIJMANS, JGM .

JOURNAL OF INHERITED METABOLIC DISEASE, 1990, 13 (03) :315-320

[5] ANALYSIS OF ORGANIC-ACIDS IN PHYSIOLOGICAL FLUIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].

BULUSU, S ;

MILLS, GA ;

WALKER, V .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1991, 14 (09) :1757-1777

[6] DIRECT SEPARATION OF 2-HYDROXY ACID ENANTIOMERS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY ON CHEMICALLY BONDED CHIRAL PHASES [J].

GUBITZ, G ;

MIHELLYES, S .

CHROMATOGRAPHIA, 1984, 19 :257-259

[7] ALPHA-KETO AND ALPHA-HYDROXY BRANCHED-CHAIN ACID INTERRELATIONSHIPS IN NORMAL HUMANS [J].

HOFFER, LJ ;

TAVEROFF, A ;

ROBITAILLE, L ;

MAMER, OA ;

REIMER, MLJ .

JOURNAL OF NUTRITION, 1993, 123 (09) :1513-1521

[8]

HOSHI S, UNPUB J LIQ CHROMATO

[9] USE OF CHARGED AND NEUTRAL CYCLODEXTRINS IN CAPILLARY ZONE ELECTROPHORESIS - ENANTIOMERIC RESOLUTION OF SOME 2-HYDROXY ACIDS [J].

NARDI, A ;

ELISEEV, A ;

BOCEK, P ;

FANALI, S .

JOURNAL OF CHROMATOGRAPHY, 1993, 638 (02) :247-253

[10] SENSITIVE ENANTIOMERIC SEPARATION OF ALIPHATIC AND AROMATIC-AMINES USING AROMATIC ANHYDRIDES AS NONCHIRAL DERIVATIZING AGENTS [J].

PAWLOWSKA, M ;

ZUKOWSKI, J ;

ARMSTRONG, DW .

JOURNAL OF CHROMATOGRAPHY A, 1994, 666 (1-2) :485-491