A LYMPHOID CELL-SPECIFIC NUCLEAR FACTOR CONTAINING C-REL-LIKE PROTEINS PREFERENTIALLY INTERACTS WITH INTERLEUKIN-6 KAPPA-B-RELATED MOTIFS WHOSE ACTIVITIES ARE REPRESSED IN LYMPHOID-CELLS

被引:74
作者
NAKAYAMA, K [1 ]
SHIMIZU, H [1 ]
MITOMO, K [1 ]
WATANABE, T [1 ]
OKAMOTO, SI [1 ]
YAMAMOTO, KI [1 ]
机构
[1] KANAZAWA UNIV,CANC RES INST,DEPT MOLEC PATHOL,13-1 TAKARAMACHI,KANAZAWA,ISHIKAWA 920,JAPAN
关键词
D O I
10.1128/MCB.12.4.1736
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The proto-oncoprotein c-Rel is a member of the nuclear factor kappa-B transcription factor family, which includes the p50 and p65 subunits of nuclear factor kappa-B. We show here that c-Rel binds to kappa-B sites as homodimers as well as heterodimers with p50. These homodimers and heterodimers show distinct DNA-binding specificities and affinities for various kappa-B motifs. In particular, the c-Rel homodimer has a high affinity for interleukin-6 (IL-6) and beta interferon kappa-B sites. In spite of its association with p50 in vitro, however, we found a lymphoid cell-specific nuclear factor in vivo that contains c-Rel but not p50 epitopes; this factor, termed IL-6-kappa-B binding factor II, appears to contain the c-Rel homodimer and preferentially recognizes several IL-6-kappa-B-related kappa-B motifs. Although it has been previously shown that the IL-6-kappa-B motif functions as a potent IL-1/tumor necrosis factor-responsive element in nonlymphoid cells, its activity was found to be repressed in lymphoid cells such as a Jurkat T-cell line. We also present evidence that IL-6-kappa-B binding factor II functions as a repressor specific for IL-6-kappa-B-related kappa-B motifs in lymphoid cells.
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页码:1736 / 1746
页数:11
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