RESISTANCE TO ANTIVIRAL INHIBITORS CAUSED BY THE MUTATION S889A IN THE HIGHLY-CONSERVED 885-GDTDS MOTIF OF THE HERPES-SIMPLEX VIRUS TYPE-1 DNA-POLYMERASE

被引：9

作者：

DORSKY, DI

PLOURDE, C

机构：

[1] Division of Infectious Diseases, Univ. of Connecticut Health Center, Farmington

来源：

VIROLOGY | 1993年 / 195卷 / 02期

关键词：

D O I：

10.1006/viro.1993.1439

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Most point substitutions in the highly-conserved 885-GDTDS motif of the HSV-1 DNA polymerase inactivate polymerase elongation activity. However, in an assay system based on expression by in vitro to transcription-translation, the mutant GDTDA (S889A) possessed wild-type elongation activity which was highly resistant to phosphonoacetic acid and acyclovir triphosphate, but retained sensitivity to aphidicolin. © 1993 Academic Press. All rights reserved.

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页码：831 / 835

页数：5

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共 31 条

[11] THE ESSENTIAL 65-KILODALTON DNA-BINDING PROTEIN OF HERPES-SIMPLEX VIRUS STIMULATES THE VIRUS-ENCODED DNA-POLYMERASE [J].

GALLO, ML ;

DORSKY, DI ;

CRUMPACKER, CS ;

PARRIS, DS .

JOURNAL OF VIROLOGY, 1989, 63 (12) :5023-5029

[12] SEQUENCE AND MAPPING ANALYSES OF THE HERPES-SIMPLEX VIRUS-DNA POLYMERASE GENE PREDICT A C-TERMINAL SUBSTRATE BINDING DOMAIN [J].

GIBBS, JS ;

CHIOU, HC ;

HALL, JD ;

MOUNT, DW ;

RETONDO, MJ ;

WELLER, SK ;

COEN, DM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (23) :7969-7973

[13] IDENTIFICATION OF AMINO-ACIDS IN HERPES-SIMPLEX VIRUS-DNA POLYMERASE INVOLVED IN SUBSTRATE AND DRUG RECOGNITION [J].

GIBBS, JS ;

CHIOU, HC ;

BASTOW, KF ;

CHENG, YC ;

COEN, DM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (18) :6672-6676

[14] COMPILATION AND ALIGNMENT OF DNA-POLYMERASE SEQUENCES [J].

ITO, J ;

BRAITHWAITE, DK .

NUCLEIC ACIDS RESEARCH, 1991, 19 (15) :4045-4057

[15] MUTAGENESIS OF CONSERVED REGION-I IN THE DNA-POLYMERASE FROM HUMAN ADENOVIRUS SEROTYPE-2 [J].

JOUNG, I ;

HORWITZ, MS ;

ENGLER, JA .

VIROLOGY, 1991, 184 (01) :235-241

[16] IN-ORGANELLO REPLICATION AND VIRAL AFFINITY OF LINEAR, EXTRACHROMOSOMAL DNA OF THE ASCOMYCETE ASCOBOLUS-IMMERSUS [J].

KEMPKEN, F ;

MEINHARDT, F ;

ESSER, K .

MOLECULAR & GENERAL GENETICS, 1989, 218 (03) :523-530

[17] COOPERATION OF EBV DNA-POLYMERASE AND EA-D(BMRF1) INVITRO AND COLOCALIZATION IN NUCLEI OF INFECTED-CELLS [J].

KIEHL, A ;

DORSKY, DI .

VIROLOGY, 1991, 184 (01) :330-340

[18] NUCLEOTIDE-SEQUENCE OF THE DNA-POLYMERASE GENE OF HERPES-SIMPLEX VIRUS TYPE-1 STRAIN ANGELOTTI [J].

KNOPF, CW .

NUCLEIC ACIDS RESEARCH, 1986, 14 (20) :8225-8226

[19] PHYSICAL MAPPING OF DRUG-RESISTANCE MUTATIONS DEFINES AN ACTIVE-CENTER OF THE HERPES-SIMPLEX VIRUS-DNA POLYMERASE ENZYME [J].

KNOPF, KW ;

KAUFMAN, ER ;

CRUMPACKER, C .

JOURNAL OF VIROLOGY, 1981, 39 (03) :746-757

[20] CRYSTAL-STRUCTURE AT 3.5 ANGSTROM RESOLUTION OF HIV-1 REVERSE-TRANSCRIPTASE COMPLEXED WITH AN INHIBITOR [J].

KOHLSTAEDT, LA ;

WANG, J ;

FRIEDMAN, JM ;

RICE, PA ;

STEITZ, TA .

SCIENCE, 1992, 256 (5065) :1783-1790

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