DEMONSTRATION OF A HETEROGENEOUS TRANSCRIPTION PATTERN OF THYROGLOBULIN MESSENGER-RNA IN HUMAN THYROID TISSUES

被引:31
作者
BERTAUX, F
NOEL, M
MALTHIERY, Y
FRAGU, P
机构
[1] FAC MED MARSEILLE, BIOCHIM MED LAB, CNRS, UA 178, F-13385 MARSEILLE 5, FRANCE
[2] FAC MED MARSEILLE, INSERM, U38, F-13385 MARSEILLE 5, FRANCE
关键词
D O I
10.1016/0006-291X(91)90148-Z
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Previous reports on human thyroglobulin (hTg) modifications in thyroid carcinomas prompted us to study hTg mRNA in thyroid adenomas and carcinomas. The quantification of hTg mRNA showed a decrease in its levels of expression in both pathological conditions which differed by a factor of 2 between adenomas and carcinomas. Furthermore, PCR was used to analyse the characteristics of hTg mRNA by amplifying 4 regions of the hTg mRNA. When applied to 2 normal, 17 benign and 13 malignant pathological tissue specimens, PCR showed no modification in the size of Tg mRNA. However, abnormal sized cDNAs appeared in all tissues with no distinction between the pathologies; the Restriction Fragment Length Polymorphism study of these cDNAs suggests the existence of alternate splicing patterns in thyroglobulin mRNAs. © 1991.
引用
收藏
页码:586 / 592
页数:7
相关论文
共 24 条
[11]   ALTERED IMMUNOREACTIVITY OF THYROGLOBULIN IN THYROID-DISEASE [J].
KIM, PS ;
DUNN, AD ;
DUNN, JT .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1988, 67 (01) :161-168
[12]   MONOCLONAL-ANTIBODIES TO THYROGLOBULIN ELUCIDATE DIFFERENCES IN PROTEIN-STRUCTURE OF THYROGLOBULIN IN HEALTHY-INDIVIDUALS AND THOSE WITH PAPILLARY ADENOCARCINOMA [J].
KOHNO, Y ;
TARUTANI, O ;
SAKATA, S ;
NAKAJIMA, H .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1985, 61 (02) :343-350
[13]  
KUSAKABE K, 1989, JPN J EXP MED, V59, P37
[14]   PRIMARY STRUCTURE OF HUMAN THYROGLOBULIN DEDUCED FROM THE SEQUENCE OF ITS 8448-BASE COMPLEMENTARY-DNA [J].
MALTHIERY, Y ;
LISSITZKY, S .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1987, 165 (03) :491-498
[15]   THYROGLOBULIN STRUCTURE AND FUNCTION - RECENT ADVANCES [J].
MALTHIERY, Y ;
MARRIQ, C ;
BERGELEFRANC, JL ;
FRANC, JL ;
HENRY, M ;
LEJEUNE, PJ ;
RUF, J ;
LISSITZKY, S .
BIOCHIMIE, 1989, 71 (02) :195-210
[16]  
MALTHIERY Y, 1989, CR SOC BIOL, V183, P33
[17]  
Maniatis T., 1982, MOL CLONING
[18]   PRIMARY STRUCTURE OF BOVINE THYROGLOBULIN DEDUCED FROM THE SEQUENCE OF ITS 8,431-BASE COMPLEMENTARY-DNA [J].
MERCKEN, L ;
SIMONS, MJ ;
SWILLENS, S ;
MASSAER, M ;
VASSART, G .
NATURE, 1985, 316 (6029) :647-651
[19]   ALTERNATIVE SPLICING MAY BE RESPONSIBLE FOR HETEROGENEITY OF THYROGLOBULIN STRUCTURE [J].
MERCKEN, L ;
SIMONS, MJ ;
BROCAS, H ;
VASSART, G .
BIOCHIMIE, 1989, 71 (02) :223-226
[20]   STRUCTURAL ORGANIZATION OF THE 5' REGION OF THE THYROGLOBULIN GENE - EVIDENCE FOR INTRON LOSS AND EXONIZATION DURING EVOLUTION [J].
PARMA, J ;
CHRISTOPHE, D ;
POHL, V ;
VASSART, G .
JOURNAL OF MOLECULAR BIOLOGY, 1987, 196 (04) :769-779