POLY(GTG)5-ASSOCIATED PROFILES OF SALMONELLA AND SHIGELLA GENOMIC DNA

被引:16
作者
DOLL, L [1 ]
MOSHITCH, S [1 ]
FRANKEL, G [1 ]
机构
[1] WEIZMANN INST SCI,DEPT MEMBRANE RES & BIOPHYS,IL-76100 REHOVOT,ISRAEL
关键词
DNA; SALMONELLA; SHIGELLA; GTG PROFILE; FINGERPRINTING; OLIGOPROBE; PLASMIDS; CLONAL STRUCTURE; EPIDEMIOLOGY;
D O I
10.1016/0923-2508(93)90211-J
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
DNA hybridization with oligonucleotide probes is a powerful technique to study population genetics and structures. The use of probes which recognize ubiquitously interspersed DNA sequences has a distinct advantage over other techniques (e.g. the analysis of patterns of restriction fragment length polymorphism) in that many independent loci can be detected simultaneously. In this communication, we investigated the use of a trinucleotide repetitive DNA oligonucleotide, poly(GTG)5, in Southern blot analysis of Salmonella serotypes and Shigella species. The strains in this study were isolated over several years from widely disparate geographic locations and can therefore be considered to represent the structure of part of the natural populations of these organisms. In most of the Salmonella serotypes, the poly(GTG)5-associated profile (GTG profile) phenotypes appeared to be clonally stable; in cases where only one isolate of a serotype was tested, the GTG profile was distinct from the others. On the other hand, when GTG profile analysis was applied to Shigella strains, each of the 12 isolates, belonging to the four Shigella species, produced a unique pattern phenotype of both the chromosome and plasmid DNA.
引用
收藏
页码:17 / 24
页数:8
相关论文
共 21 条
  • [11] HYPERVARIABLE MINISATELLITE REGIONS IN HUMAN DNA
    JEFFREYS, AJ
    WILSON, V
    THEIN, SL
    [J]. NATURE, 1985, 314 (6006) : 67 - 73
  • [12] MOLECULAR EPIDEMIOLOGY OF VIBRIO-CHOLERAE IN THE UNITED-STATES GULF-COAST
    KAPER, JB
    BRADFORD, HB
    ROBERTS, NC
    FALKOW, S
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1982, 16 (01) : 129 - 134
  • [13] LIDOR H, 1982, J CLIN MICROBIOL, V15, P761
  • [14] MAHAR KO, 1986, AM J TROP MED HYG, V35, P831
  • [15] SUMMARY OF A WORKSHOP ON THE CLONE CONCEPT IN THE EPIDEMIOLOGY, TAXONOMY, AND EVOLUTION OF THE ENTEROBACTERIACEAE AND OTHER BACTERIA
    ORSKOV, F
    ORSKOV, I
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1983, 148 (02) : 346 - 357
  • [16] CLONAL NATURE OF SALMONELLA-TYPHI AND ITS GENETIC RELATEDNESS TO OTHER SALMONELLAE AS SHOWN BY MULTILOCUS ENZYME ELECTROPHORESIS, AND PROPOSAL OF SALMONELLA-BONGORI COMB NOV
    REEVES, MW
    EVINS, GM
    HEIBA, AA
    PLIKAYTIS, BD
    FARMER, JJ
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (02) : 313 - 320
  • [17] EVOLUTIONARY GENETIC-RELATIONSHIPS OF CLONES OF SALMONELLA SEROVARS THAT CAUSE HUMAN TYPHOID AND OTHER ENTERIC FEVERS
    SELANDER, RK
    BELTRAN, P
    SMITH, NH
    HELMUTH, R
    RUBIN, FA
    KOPECKO, DJ
    FERRIS, K
    TALL, BD
    CRAVIOTO, A
    MUSSER, JM
    [J]. INFECTION AND IMMUNITY, 1990, 58 (07) : 2262 - 2275
  • [18] DETECTION OF SPECIFIC SEQUENCES AMONG DNA FRAGMENTS SEPARATED BY GEL-ELECTROPHORESIS
    SOUTHERN, EM
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1975, 98 (03) : 503 - +
  • [19] A BROAD-SPECTRUM PROBE FOR MOLECULAR EPIDEMIOLOGY OF BACTERIA - RIBOSOMAL-RNA
    STULL, TL
    LIPUMA, JJ
    EDLIND, TD
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1988, 157 (02) : 280 - 286
  • [20] HYPERVARIABILITY OF SIMPLE SEQUENCES AS A GENERAL SOURCE FOR POLYMORPHIC DNA MARKERS
    TAUTZ, D
    [J]. NUCLEIC ACIDS RESEARCH, 1989, 17 (16) : 6463 - 6471