STIMULATION OF THE DNA-DEPENDENT PROTEIN-KINASE BY RNA-POLYMERASE-II TRANSCRIPTIONAL ACTIVATOR PROTEINS

被引:63
作者
PETERSON, SR
JESCH, SA
CHAMBERLIN, TN
DVIR, A
RABINDRAN, SK
WU, C
DYNAN, WS
机构
[1] UNIV COLORADO,DEPT CHEM & BIOCHEM,BOULDER,CO 80309
[2] NCI,BIOCHEM LAB,BETHESDA,MD 20892
关键词
D O I
10.1074/jbc.270.3.1449
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The DNA dependent protein kinase (DNA-PK) phosphorylates RNA polymerase II and a number of transcription factors. We now show that the activity of DNA-PK is directly stimulated by certain transcriptional activator proteins, including the human heat shock transcription factor 1 (HSF1) and a transcriptionally active N-terminal 147 amino acid GAL4 derivative. Stimulation of DNA-PK activity required specific sequences in the activator proteins outside the minimal DNA binding domains. The stimulation of DNA-PK activity also required DNA and was greater with DNA containing relevant activator binding sites. Comparison of different HSF binding fragments showed that optimal stimulation occurred when two HSF binding sites were present. Stimulation with HSF and GALE was synergistic with Ku protein, another regulator of DNA-PK activity. DNA-PK is tightly associated with the transcriptional template, and an increase in its activity could potentially influence transcription through the phosphorylation of proteins associated with the transcription complex.
引用
收藏
页码:1449 / 1454
页数:6
相关论文
共 43 条
[31]   PROTEIN TRAFFIC ON THE HEAT-SHOCK PROMOTER - PARKING, STALLING, AND TRUCKING ALONG [J].
LIS, J ;
WU, C .
CELL, 1993, 74 (01) :1-4
[32]  
LIU SH, 1993, J BIOL CHEM, V268, P21147
[33]   MYB DNA-BINDING INHIBITED BY PHOSPHORYLATION AT A SITE DELETED DURING ONCOGENIC ACTIVATION [J].
LUSCHER, B ;
CHRISTENSON, E ;
LITCHFIELD, DW ;
KREBS, EG ;
EISENMAN, RN .
NATURE, 1990, 344 (6266) :517-522
[34]   PHOSPHORYLATION OF RNA-POLYMERASE-II C-TERMINAL DOMAIN AND TRANSCRIPTIONAL ELONGATION [J].
OBRIEN, T ;
HARDIN, S ;
GREENLEAF, A ;
LIS, JT .
NATURE, 1994, 370 (6484) :75-77
[35]   DNA-BINDING PROVIDES A SIGNAL FOR PHOSPHORYLATION OF THE RNA POLYMERASE-II HEPTAPEPTIDE REPEATS [J].
PETERSON, SR ;
DVIR, A ;
ANDERSON, CW ;
DYNAN, WS .
GENES & DEVELOPMENT, 1992, 6 (03) :426-438
[36]  
PETERSON SR, 1993, THESIS U COLORADO
[37]   MOLECULAR-CLONING AND EXPRESSION OF A HUMAN HEAT-SHOCK FACTOR, HSF1 [J].
RABINDRAN, SK ;
GIORGI, G ;
CLOS, J ;
WU, C .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (16) :6906-6910
[38]   ACTIVATION OF HEAT-SHOCK GENE-TRANSCRIPTION BY HEAT-SHOCK FACTOR-I INVOLVES OLIGOMERIZATION, ACQUISITION OF DNA-BINDING ACTIVITY, AND NUCLEAR-LOCALIZATION AND CAN OCCUR IN THE ABSENCE OF STRESS [J].
SARGE, KD ;
MURPHY, SP ;
MORIMOTO, RI .
MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (03) :1392-1407
[39]   DIRECT AND SELECTIVE BINDING OF AN ACIDIC TRANSCRIPTIONAL ACTIVATION DOMAIN TO THE TATA-BOX FACTOR TFIID [J].
STRINGER, KF ;
INGLES, CJ ;
GREENBLATT, J .
NATURE, 1990, 345 (6278) :783-786
[40]   DOUBLE-STRANDED DNA INDUCES THE PHOSPHORYLATION OF SEVERAL PROTEINS INCLUDING THE 90 000 MOL WT HEAT-SHOCK PROTEIN IN ANIMAL-CELL EXTRACTS [J].
WALKER, AI ;
HUNT, T ;
JACKSON, RJ ;
ANDERSON, CW .
EMBO JOURNAL, 1985, 4 (01) :139-145