ENZYMATIC-SYNTHESIS OF N-LINKED AND O-LINKED GLYCOPEPTIDES

被引：88

作者：

WONG, CH

SCHUSTER, M

WANG, P

SEARS, P

机构：

[1] Department of Chemistry, The Scripps Research Institute, La Jolla, California 92037

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 1993年 / 115卷 / 14期

关键词：

D O I：

10.1021/ja00067a001

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This paper describes the study of kinetically controlled enzymatic coupling of N- and O-glycopeptide fragments using subtilisin BPN' and two of its variants developed for use in high concentrations of dimethylformamide and in aqueous solution, respectively. Glycosyl amino acids were exploited as the P1, P2, P3, P2', P2', or P3' residue in the enzymatic coupling. Glycosyltransferase-mediated glycosylation of the glycopeptide fragments obtained prior to or after enzymatic peptide bond formation is demonstrated.

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页码：5893 / 5901

页数：9

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