CLONING AND ANALYSIS OF CHICKEN ACETYLCHOLINESTERASE TRANSCRIPTS FROM MUSCLE AND BRAIN

被引：23

作者：

RANDALL, WR ^{[1
]}

RIMER, M ^{[1
]}

GOUGH, NR ^{[1
]}

机构：

[1] UNIV MARYLAND,SCH MED,CTR MED BIOTECHNOL,BALTIMORE,MD 21201

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1994年 / 1218卷 / 03期

关键词：

ACETYLCHOLINESTERASE; CATALYTIC SUBUNIT; CLONING; AMINO ACID SEQUENCE; CDNA; (CHICKEN);

D O I：

10.1016/0167-4781(94)90204-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated cDNA clones from an embryonic chicken muscle cDNA library which encodes the complete catalytic T subunit of acetylcholinesterase. The deduced polypeptide comprises 767 amino acids, shows similar to 60% homology to acetylcholinesterases from other vertebrates and contains a 155 amino acid sequence inserted into the middle of the peptide which is unique to the chicken enzyme. Northern blots of embryonic chicken muscle and adult brain show three transcripts approximately 4.5, 5.5, and 6.0 kb hybridizing to a cDNA fragment of AChE. The 6.0 kb transcript is expressed transiently in embryonic muscle and is a major transcript in adult brain.

引用

页码：453 / 456

页数：4

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