THE REQUIREMENT OF ARCA AND FNR FOR PEAK EXPRESSION OF THE CYD OPERON IN ESCHERICHIA-COLI UNDER MICROAEROBIC CONDITIONS

被引：104

作者：

FU, HA ^{[1
]}

IUCHI, S ^{[1
]}

LIN, ECC ^{[1
]}

机构：

[1] HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOLEC GENET,200 LONGWOOD AVE,BOSTON,MA 02115

来源：

MOLECULAR & GENERAL GENETICS | 1991年 / 226卷 / 1-2期

关键词：

MICROAEROBIOSIS; CYD; ARCA; FNR; ESCHERICHIA-COLI;

D O I：

10.1007/BF00273605

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transcriptional regulation of cyd, encoding the cytochrome d complex for O2 scavenging, was studied in Escherichia coli by monitoring phi(cyd-lac) expression under atmospheres containing 0-21% O2. Peak expression of this operon occurred under microaerobic conditions. Mutations in arcA (a trans-acting regulatory gene involved in aerobic respiration) greatly lowered phi(cyd-lac) expression under all conditions. Mutations in fnr (a trans-acting regulatory gene involved in anaerobic respiration) shifted the pattern of phi(cyd-lac) expression, lowering the microaerobic and aerobic expression. In an arcA-fnr double mutant, phi(cyd-lac) expression became insignificant, irrespective of the availability of O2. It thus appears that the expression of the phi(cyd-lac) operon is under dual control by the two pleiotropic activators, ArcA and Fnr, which interact to give the peak microaerobic expression.

引用

页码：209 / 213

页数：5

共 24 条

[1] THE AEROBIC RESPIRATORY-CHAIN OF ESCHERICHIA-COLI
ANRAKU, Y
GENNIS, RB
[J]. TRENDS IN BIOCHEMICAL SCIENCES, 1987, 12 (07) : 262 - 266
[2] MUTATION LEADING TO TOTAL LACK OF NITRITE REDUCTASE-ACTIVITY IN ESCHERICHIA-COLI-K-12
CHIPPAUX, M
GIUDICI, D
ABOUJAOUDE, A
CASSE, F
PASCAL, MC
[J]. MOLECULAR & GENERAL GENETICS, 1978, 160 (02): : 225 - 229
[3] MUTATIONS IN THE ESCHERICHIA-COLI FNR AND TGT GENES - CONTROL OF MOLYBDATE REDUCTASE-ACTIVITY AND THE CYTOCHROME-D COMPLEX BY FNR
FREY, B
JANEL, G
MICHELSEN, U
KERSTEN, H
[J]. JOURNAL OF BACTERIOLOGY, 1989, 171 (03) : 1524 - 1530
[4] REGULATION OF EXPRESSION OF THE CYTOCHROME-D TERMINAL OXIDASE IN ESCHERICHIA-COLI IS TRANSCRIPTIONAL
GEORGIOU, CD
DUEWEKE, TJ
GENNIS, RB
[J]. JOURNAL OF BACTERIOLOGY, 1988, 170 (02) : 961 - 966
[5] THE NARL GENE-PRODUCT ACTIVATES THE NITRATE REDUCTASE OPERON AND REPRESSES THE FUMARATE REDUCTASE AND TRIMETHYLAMINE N-OXIDE REDUCTASE OPERONS IN ESCHERICHIA-COLI
IUCHI, S
LIN, ECC
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (11) : 3901 - 3905
[6] 3 CLASSES OF ESCHERICHIA-COLI MUTANTS SELECTED FOR AEROBIC EXPRESSION OF FUMARATE REDUCTASE
IUCHI, S
KURITZKES, DR
LIN, ECC
[J]. JOURNAL OF BACTERIOLOGY, 1986, 168 (03) : 1415 - 1421
[7] REQUIREMENT FOR TERMINAL CYTOCHROMES IN GENERATION OF THE AEROBIC SIGNAL FOR THE ARC REGULATORY SYSTEM IN ESCHERICHIA-COLI - STUDY UTILIZING DELETIONS AND LAC FUSIONS OF CYO AND CYD
IUCHI, S
CHEPURI, V
FU, HA
GENNIS, RB
LIN, ECC
[J]. JOURNAL OF BACTERIOLOGY, 1990, 172 (10) : 6020 - 6025
[8] THE ARCB GENE OF ESCHERICHIA-COLI ENCODES A SENSOR-REGULATOR PROTEIN FOR ANAEROBIC REPRESSION OF THE ARC MODULON
IUCHI, S
FUJIWARA, T
LIN, ECC
[J]. MOLECULAR MICROBIOLOGY, 1990, 4 (05) : 715 - 727
[9] ARCA (DYE), A GLOBAL REGULATORY GENE IN ESCHERICHIA-COLI MEDIATING REPRESSION OF ENZYMES IN AEROBIC PATHWAYS
IUCHI, S
LIN, ECC
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (06) : 1888 - 1892
[10] REGULATION OF ESCHERICHIA-COLI FUMARATE REDUCTASE (FRDABCD) OPERON EXPRESSION BY RESPIRATORY ELECTRON-ACCEPTORS AND THE FNR GENE-PRODUCT
JONES, HM
GUNSALUS, RP
[J]. JOURNAL OF BACTERIOLOGY, 1987, 169 (07) : 3340 - 3349

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