INHIBITION OF CRH-41 RELEASE BY SUBSTANCE-P, BUT NOT SUBSTANCE-K, FROM THE RAT HYPOTHALAMUS INVITRO

被引：42

作者：

FARIA, M ^{[1
]}

NAVARRA, P ^{[1
]}

TSAGARAKIS, S ^{[1
]}

BESSER, GM ^{[1
]}

GROSSMAN, AB ^{[1
]}

机构：

[1] ST BARTHOLOMEWS HOSP,DEPT ENDOCRINOL,LONDON EC1A 7BE,ENGLAND

来源：

BRAIN RESEARCH | 1991年 / 538卷 / 01期

关键词：

CRH-41; HYPOTHALAMUS; SUBSTANCE-P; SUBSTANCE-K; TACHYKININ; ADRENOCORTICOTROPIC HORMONE;

D O I：

10.1016/0006-8993(91)90378-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Substance P is one of a series of tachykinins which is present throughout the central nervous system, and has potent effects on neuroendocrine function. Recent studies have suggested that it inhibits pituitary-adrenal activity at a site above the level of the pituitary. We have therefore used a well-validated rat hypothalamic incubation system to investigate the effects of substance P on the release of the principal corticotrophin-releasing hormone, CRH-41. Substance P caused a dose-dependent inhibition of the 28-mM KCl-stimulated release of CRH-41, with a maximum effect at 100 nM (P < 0.01). This effect was attenuated by 10-mu-M of the substance P antagonist (D-Pro2, -D-Trp7,9)-substance P. No statistically significant effect of substance K was seen at 1 or 100 nM. Substance P, at a dose of 100 nM, did not alter the 28-mM KCl-stimulated release of CRH-41 from isolated median eminences in vitro. It is concluded that substance P is a potent inhibitor of the stimulated release of CRH-41, probably acting at a site within the hypothalamic paraventricular nucleus.

引用

页码：76 / 78

页数：3