3-DIMENSIONAL TRANSFER-FUNCTION ANALYSIS OF THE TOMOGRAPHIC CAPABILITY OF A CONFOCAL FLUORESCENCE MICROSCOPE

被引：41

作者：

NAKAMURA, O ^{[1
]}

KAWATA, S ^{[1
]}

机构：

[1] OSAKA UNIV,DEPT APPL PHYS,SUITA,OSAKA 565,JAPAN

来源：

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION | 1990年 / 7卷 / 03期

关键词：

D O I：

10.1364/JOSAA.7.000522

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The three-dimensional optical transfer function of a confocal fluorescence microscope is derived. It has no missing cone and provides tomographic images of the sample. The derivation is based on the dispersion equation of spherical waves and the diffraction limitation by an objective lens. Experimental results are shown to verify the derivation. © 1990 Optical Society of America.

引用

页码：522 / 526

页数：5

共 18 条

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