INTRACELLULAR MG-2+ MODULATES THE A-CURRENT AND ITS BLOCKAGE BY CATECHOL IN ISOLATED LYMNAEA NEURONS

被引：4

作者：

ERDELYI, L ^{[1
]}

ILYIN, VI ^{[1
]}

LOZOVAYA, NA ^{[1
]}

VULFIUS, CA ^{[1
]}

机构：

[1] ACAD SCI USSR,INST BIOL PHYS,PUSHCHINO,USSR

来源：

NEUROSCIENCE LETTERS | 1990年 / 117卷 / 1-2期

关键词：

A-current; Catechol-induced blockage; Intracellular Mg[!sup]2+[!/sup; Lymnaea neuron; Voltage clamp;

D O I：

10.1016/0304-3940(90)90126-T

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The effects of intracellular Mg2+ (2-8 mM) upon the transient outward current (the A-current) under normal conditions and under catechol-induced blockage were studied in molluscan neurons by using the voltage-clamp and intracellular dialysis techniques. Identified giant Lymnaea stagnalis L. neurons were investigated at room temperature (20-22°C). When applied intracellularly, Mg2+ caused both time- and dose-dependent shifts of the voltage dependence of the steady-state activation and inactivation of the A-current to more negative membrane potentials. Upon external application, catechol suppressed (5-6 mM) or eliminated (9-10 mM) the A-currents, slowed down the current decay and shifted the activation and inactivation curves to more positive membrane voltages. Intracellular Mg2+ decreased the blocking ability of extracellularly applied catechol, whereas catechol antagonized the Mg2+-induced negative shift of the steady-state activation and inactivation curves of the A-currents. © 1990.

引用

页码：99 / 104

页数：6

共 14 条

[11] MAGNESIUM GATES GLUTAMATE-ACTIVATED CHANNELS IN MOUSE CENTRAL NEURONS [J].