FEASIBILITY OF CAPILLARY ZONE ELECTROPHORESIS WITH SUPPRESSION OF ELECTROENDOSMOTIC FLOW IN COMPLETELY CLOSED SYSTEMS

被引：46

作者：

VERHEGGEN, TPEM ^{[1
]}

SCHOOTS, AC ^{[1
]}

EVERAERTS, FM ^{[1
]}

机构：

[1] EINDHOVEN UNIV TECHNOL,FAC CHEM TECHNOL,INSTRUMENTAL ANAL LAB,5600 MB EINDHOVEN,NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY | 1990年 / 503卷 / 01期

关键词：

D O I：

10.1016/S0021-9673(01)81506-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Instrumental aspects of capillary zone electrophoresis in closed systems are reported. The combination of fixed-volume sample introduction, utilization of double-beam UV absorbance detection and the use of fixed membranes for the separation of the electrode reservoirs from the separation capillary proved to be a reliable instrumental configuration for capillary zone electrophoresis. Dispersion of eluted zones was studied as a function of capillary length. Repeatabilities of migration times and peak areas were determined. Several examples of separations of mixtures of organic acids and nucleotides are given. The separation of diluted and ultrafiltrated serum from patients with chronic renal failure proved to be promising. © 1990.

引用

页码：245 / 255

页数：11

共 24 条

[1] HIGH-PERFORMANCE SODIUM DODECYL-SULFATE POLYACRYLAMIDE-GEL CAPILLARY ELECTROPHORESIS OF PEPTIDES AND PROTEINS [J].

COHEN, AS ;

KARGER, BL .

JOURNAL OF CHROMATOGRAPHY, 1987, 397 :409-417

[2]

Everaerts F. M., 1970, SCI TOOLS, V17, P25

[3]

EVERAERTS FM, 1989, HRC-J HIGH RES CHROM, V12, P28

[4]

EVERAERTS FM, 1976, ISOTACHOPHORESIS

[5] CAPILLARY ELECTROPHORESIS [J].

EWING, AG ;

WALLINGFORD, RA ;

OLEFIROWICZ, TM .

ANALYTICAL CHEMISTRY, 1989, 61 (04) :A292-+

[6] CAPILLARY ZONE ELECTROPHORESIS - QUANTITATIVE STUDY OF THE EFFECTS OF SOME DISPERSIVE PROCESSES ON THE SEPARATION EFFICIENCY [J].

FORET, F ;

DEML, M ;

BOCEK, P .

JOURNAL OF CHROMATOGRAPHY, 1988, 452 :601-613

[7] GENERATION OF VARIANCE, THEORETICAL PLATES, RESOLUTION, AND PEAK CAPACITY IN ELECTROPHORESIS AND SEDIMENTATION [J].

GIDDINGS, JC .

SEPARATION SCIENCE, 1969, 4 (03) :181-&

[8] HIGH-RESOLUTION SEPARATIONS BASED ON ELECTROPHORESIS AND ELECTROOSMOSIS [J].

JORGENSON, JW ;

LUKACS, KD .

JOURNAL OF CHROMATOGRAPHY, 1981, 218 (1-3) :209-216

[9] MINIATURIZATION IN PRESSURE AND ELECTROENDOSMOTICALLY DRIVEN LIQUID-CHROMATOGRAPHY - SOME THEORETICAL CONSIDERATIONS [J].

KNOX, JH ;

GRANT, IH .

CHROMATOGRAPHIA, 1987, 24 :135-143

[10]

Kohlraush F., 1897, ANN PHYS CHEM, V62, P209

← 1 2 3 →