DIFFERENTIAL-EFFECTS OF CALCIUM-CHANNEL ANTAGONISTS ON TITYUSTOXIN AND OUABAIN-INDUCED RELEASE OF [H-3] ACETYLCHOLINE FROM BRAIN CORTICAL SLICES

被引：22

作者：

CASALI, TAA

GOMEZ, RS

MORAESSANTOS, T

GOMEZ, MV

机构：

[1] UFMG BELO,ICB,DEPT FARMACOL,GERAIS,BRAZIL

[2] UFMG BELO,FAC MED,DEPT CIRURG,GERAIS,BRAZIL

[3] UFMG BELO,FAC FARM,DEPT ALIMENTOS,GERAIS,BRAZIL

来源：

NEUROPHARMACOLOGY | 1995年 / 34卷 / 06期

关键词：

TITYUSTOXIN; OUABAIN; CALCIUM CHANNEL ANTAGONISTS; ACETYLCHOLINE OMEGA-CONOTOXINS; OMEGA-CONOTOXIN MVIIC; OMEGA-AGAIVA;

D O I：

10.1016/0028-3908(95)00019-3

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

In this paper, the effect of calcium channel blockers on acetylcholine release induced by tityustoxin and ouabain in rat brain cortical slices is described. Cadmium, a non-specific blocker of calcium channels, inhibited the release of ACh induced by tityustoxin. L-type calcium channel blockers (verapamil, nifedipine and diltiazen) had no effect on the release of ACh induced by tityustoxin. The release of ACh was also unaffected by nickel, a T-type calcium channel blocker, and the conotoxins GVIA and MVIIC, blockers of N and Q-type calcium channels. Agatoxin IVA, a specific blocker of the P-type calcium channel, inhibited the release of ACh induced by tityustoxin by 50%. The spontaneous release of ACh as well as ouabain-induced release of ACh was unaffected by any of the calcium channel blockers studied. It is concluded that ACh release induced by tityustoxin is mediated by Ca2+ influx via P-type calcium channels.

引用

页码：599 / 603

页数：5

共 29 条

[1]

Adam-Vizi, Ligetti, Release of acetylcholine from rat brain synaptosomes by various agents in the absence of external calcium ions, J. Physiol. Lond., 353, (1984)

[2]

Adam Vizi, Ligetti, Calcium uptake of rat brain synaptosomes as a function of membrane potential under different depolarizing conditions., J Physiol, 372, pp. 363-377, (1986)

[3]

Brosemer, Effects of inhibitors of Na<sup>+</sup>K<sup>+</sup>ATPase on the membrane potentials and neurotransmitter efflux in rat brain slices, Brain Res., 334, pp. 125-137, (1985)

[4]

Fox, Nowycky, Tsien, Kinetic and pharmacological properties distinguishing three types of calcium currents in chick sensory neurons, J. Physiol. Lond., 394, pp. 149-172, (1987)

[5]

Gomez, Diniz, Separation of toxic components from the Brazilian scorpion Tityus serrulatus venom, Mem. Int. Butantan. Symp. Int., 33, pp. 899-902, (1966)

[6]

Gomez, Dai, Diniz, Effects of scorpion venom tityustoxin on the release of acetylcholine from incubated slides of rat brain, J. Neurochem., 20, pp. 1051-1061, (1973)

[7]

Gomez, Diniz, Barbosa, A comparison of the effect of scorpion venom tityustoxin and ouabain on the release of acetylcholine from incubated slices of rat brain, J. Neurochem., 24, pp. 331-336, (1975)

[8]

Henriques, Gomez, The effect of scorpion venom tityustoxin on the uptake of calcium in synaptosomes, Brain Res. Bull., 7, pp. 255-259, (1981)

[9]

Hille, Ionic Channels of Excitable Membranes, pp. 109-119, (1992)

[10]

Katz, Miledi, The Croonian Lecture: The Transmission of Impulses from Nerve to Muscle, and the Subcellular Unit of Synaptic Action, Proceedings of the Royal Society B: Biological Sciences, 155, pp. 455-477, (1965)

← 1 2 3 →