IDENTIFICATION OF POL-RELATED GENE-PRODUCTS OF HUMAN FOAMY VIRUS

被引:30
作者
NETZER, KO
SCHLIEPHAKE, A
MAURER, B
WATANABE, R
AGUZZI, A
RETHWILM, A
机构
[1] INST VIROL & IMMUNOBIOL,VERSBACHERSTR 7,W-8700 WURZBURG,GERMANY
[2] SOKA UNIV,INST LIFE SCI,HACHIOJI,TOKYO 192,JAPAN
[3] RES INST MOLEC PATHOL,A-1030 VIENNA,AUSTRIA
关键词
D O I
10.1006/viro.1993.1039
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Human foamy virus pol gene fragments were molecularly cloned into a procaryotic expression vector. The expression pattern of the cloned fragments and nucleotide sequence analysis of the 5′ pol gene region revealed that in HFV the protease (PR) is located in the pol open reading frame. Purified recombinant proteins were used to generate antibodies in rats. In immunoblot assay, using infected cells as antigen, a precursor protein with an apparent molecular mass (Mr) of 127K was identified by antibodies directed against the reverse transcriptase (RT), RNaseH, or integrase (IN) domains of pol. With concentrated virus as antigen, the RT and RNaseH antibodies recognized a protein of 80K, the IN antiserum recognized a protein of 40K, and the PR antiserum detected a protein of approximately 10K. © 1993 Academic Press, Inc.
引用
收藏
页码:336 / 338
页数:3
相关论文
共 28 条
[11]   IMMUNOENZYMATIC DETECTION OF EXPRESSED GENE FRAGMENTS CLONED IN THE LAC-Z GENE OF ESCHERICHIA-COLI [J].
KOENEN, M ;
RUTHER, U ;
MULLERHILL, B .
EMBO JOURNAL, 1982, 1 (04) :509-512
[12]   SEQUENCE-ANALYSIS OF THE SIMIAN FOAMY VIRUS TYPE-1 GENOME [J].
KUPIEC, JJ ;
KAY, A ;
HAYAT, M ;
RAVIER, R ;
PERIES, J ;
GALIBERT, F .
GENE, 1991, 101 (02) :185-194
[13]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[14]   HUMAN SPUMAVIRUS ANTIBODIES IN SERA FROM AFRICAN PATIENTS [J].
MAHNKE, C ;
KASHAIYA, P ;
ROSSLER, J ;
BANNERT, H ;
LEVIN, A ;
BLATTNER, WA ;
DIETRICH, M ;
LUANDE, J ;
LOCHELT, M ;
FRIEDMANKIEN, AE ;
KOMAROFF, AL ;
LOH, PC ;
WESTARP, ME ;
FLUGEL, RM .
ARCHIVES OF VIROLOGY, 1992, 123 (3-4) :243-253
[15]   ANALYSIS OF THE PRIMARY STRUCTURE OF THE LONG TERMINAL REPEAT AND THE GAG AND POL GENES OF THE HUMAN SPUMARETROVIRUS [J].
MAURER, B ;
BANNERT, H ;
DARAI, G ;
FLUGEL, RM .
JOURNAL OF VIROLOGY, 1988, 62 (05) :1590-1597
[16]   GENOMIC ORGANIZATION OF THE HUMAN SPUMARETROVIRUS AND ITS RELATEDNESS TO AIDS AND OTHER RETROVIRUSES [J].
MAURER, B ;
FLUGEL, RM .
AIDS RESEARCH AND HUMAN RETROVIRUSES, 1988, 4 (06) :467-473
[17]   THE PREVALENCE OF NATURALLY-OCCURRING ANTIBODIES TO HUMAN SYNCYTIAL VIRUS IN EAST-AFRICAN POPULATIONS [J].
MULLER, HK ;
BALL, G ;
EPSTEIN, MA ;
ACHONG, BG ;
LENOIR, G ;
LEVIN, A .
JOURNAL OF GENERAL VIROLOGY, 1980, 47 (APR) :399-406
[18]   IDENTIFICATION OF THE MAJOR IMMUNOGENIC STRUCTURAL PROTEINS OF HUMAN FOAMY VIRUS [J].
NETZER, KO ;
RETHWILM, A ;
MAURER, B ;
TERMEULEN, V .
JOURNAL OF GENERAL VIROLOGY, 1990, 71 :1237-1241
[19]   GENOMIC ORGANIZATION AND EXPRESSION OF SIMIAN FOAMY VIRUS TYPE-3 (SFV-3) [J].
RENNE, R ;
FRIEDL, E ;
SCHWEIZER, M ;
FLEPS, U ;
TUREK, R ;
NEUMANNHAEFELIN, D .
VIROLOGY, 1992, 186 (02) :597-608
[20]   MOLECULAR-CLONING OF THE GENOME OF HUMAN SPUMARETROVIRUS [J].
RETHWILM, A ;
DARAI, G ;
ROSEN, A ;
MAURER, B ;
FLUGEL, RM .
GENE, 1987, 59 (01) :19-28