NUCLEOSIDES AND NUCLEOTIDES .135. DNA DUPLEX AND TRIPLER FORMATION AND RESISTANCE TO NUCLEOLYTIC DEGRADATION OF OLIGODEOXYNUCLEOTIDES CONTAINING SYN-NORSPERMIDINE AT THE 5-POSITION OF 2'-DEOXYURIDINE

被引：41

作者：

NARA, H ^{[1
]}

ONO, A ^{[1
]}

MATSUDA, A ^{[1
]}

机构：

[1] HOKKAIDO UNIV,FAC PHARMACEUT SCI,KITA KU,SAPPORO,HOKKAIDO 060,JAPAN

来源：

BIOCONJUGATE CHEMISTRY | 1995年 / 6卷 / 01期

关键词：

D O I：

10.1021/bc00031a005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel 2'-deoxyuridine analogue with syn-norspermidine at the 5-position, 5-[4-[N,N-bis(3-amino-propyl)amino]butyl]-2'-deoxyuridine (1), has been synthesized from 5-iodo-2'-deoxyuridine. This nucleoside 1 was incorporated into heptadecadeoxynucleotides 5'-d[1(MT)(8)]-3' and 5'-[(TM)(4)1(MT)(4)]-3' (M = 5-methyl-2'-deoxycytidine). The triamine group stabilized duplex and tripler formation of the heptadecadeoxynucleotides with a complementary strand and a target duplex, respectively. The oligonucleotides containing 1 were more resistant to nuclease P1 and snake venom phosphodiesterase than an unmodified heptadecadeoxynucleotide, 5'-d[T(MT)(8)]-3'.

引用

页码：54 / 61

页数：8