STUDIES ON INTERLEUKIN-2 RECEPTOR EXPRESSION AND IL-2 PRODUCTION BY MURINE T-CELL LYMPHOMAS

In order to study the possible role of the T-lymphocyte growth factor. Interleukin 2 (IL 2), and/or of the IL-2 receptor in the autonomous growth of leukemic cells. Mouse cell lines (15) of various etiology were analyzed for IL-2 receptor expression and for the capacity to secrete IL-2. Several but not all of the cell lines tested were IL-2 receptor positive. The cells constitutively expressing IL-2 receptors at their surface could not be stimulated IL-2. Cell producing and secreting IL-2 did not express detectable amounts of IL-2 receptors at their surface. Proliferation of the leukemic cells was independent of exogenous IL-2. The monoclonal anti-IL-2 receptor antibody AMT-13 inhibited IL-2 dependent proliferation of activated normal T-lymphocytes but failed to inhibit the growth of IL-2 receptor expressing leukemia cells. The results argue against the autocrine stimulation hypothesis but do not exclude the possibility of involvement of functionally altered IL-2 receptors on autonomous cell growth.