A NEW REGULATORY ELEMENT THAT AUGMENTS THE TAX-DEPENDENT ENHANCER OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-1 AND CLONING OF CDNAS ENCODING ITS BINDING-PROTEINS

被引:42
作者
TANIMURA, A
TESHIMA, H
FUJISAWA, JI
YOSHIDA, M
机构
[1] UNIV TOKYO,INST MED SCI,DEPT CELLULAR & MOLEC BIOL,MINATO KU,TOKYO 108,JAPAN
[2] UNIV TOKYO,DEPT AGR CHEM,BIOL CHEM LAB,TOKYO 108,JAPAN
关键词
D O I
10.1128/JVI.67.9.5375-5382.1993
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The Tax protein of human T-cell leukemia virus type 1 (HTLV-1) trans activates the 21-bp enhancer of HTLV-1. A sequence of more than two copies of the 21-bp enhancer is efficiently activated by Tax, but one copy is not activated extensively. Another sequence (TRE-2, positions - 163 to - 117) adjacent to the 21-bp enhancer in the long terminal repeat of HTLV-1 can enhance a single copy of the 21-bp enhancer activity in trans activation by Tax. This sequence contains motifs related to the Ets- and NF-KB-binding sequences, but mutations at these sites indicated that neither is responsive to cooperation with the 21-bp enhancer. A deletion mutation of TRE-2 identified 25 bases at positions - 158 to - 134 (TRE-2S) as an essential sequence, and TRE-2S was sufficient to give maximum cooperation with one copy of the 21-bp enhancer in trans activation by Tax protein. Using TRE-2S as a probe, we screened a cDNA library of HUT102 cells by the Southwestern (DNA-protein) procedure and isolated two cDNA clones, THP-1 and -2. These two clones encode TRE-2S-binding proteins, and they differ by only an extra 17 amino acids in THP-2. Both THP proteins contain five zinc finger motifs which are strikingly similar to those of the GLI family, an amplified gene product in glyoma cells. The binding site of THP-1 and -2 was GAACCACCCA in TRE-2S, which is highly homologous to the GLI-binding site. These results suggest that binding of THP to TRE-2S may be involved in cooperation with one copy of the 21-bp enhancer in responding to Tax trans activation.
引用
收藏
页码:5375 / 5382
页数:8
相关论文
共 47 条
[1]   BINDING OF THE HTLV-I TAX 1 TRANSACTIVATOR TO THE INDUCIBLE 21 BP ENHANCER IS MEDIATED BY THE CELLULAR FACTOR-HEB1 [J].
BERAUD, C ;
LOMBARDPLATET, G ;
MICHAL, Y ;
JALINOT, P .
EMBO JOURNAL, 1991, 10 (12) :3795-3803
[2]   POTENTIAL METAL-BINDING DOMAINS IN NUCLEIC-ACID BINDING-PROTEINS [J].
BERG, JM .
SCIENCE, 1986, 232 (4749) :485-487
[3]   MYB PROTEIN BINDS TO MULTIPLE SITES IN THE HUMAN T-CELL LYMPHOTROPIC VIRUS TYPE-1 LONG TERMINAL REPEAT AND TRANSACTIVATES LTR-MEDIATED EXPRESSION [J].
BOSSELUT, R ;
LIM, F ;
ROMOND, PC ;
FRAMPTON, J ;
BRADY, J ;
GHYSDAEL, J .
VIROLOGY, 1992, 186 (02) :764-769
[4]   THE PRODUCT OF THE C-ETS-1 PROTOONCOGENE AND THE RELATED ETS2 PROTEIN ACT AS TRANSCRIPTIONAL ACTIVATORS OF THE LONG TERMINAL REPEAT OF HUMAN T-CELL LEUKEMIA-VIRUS HTLV-1 [J].
BOSSELUT, R ;
DUVALL, JF ;
GEGONNE, A ;
BAILLY, M ;
HEMAR, A ;
BRADY, J ;
GHYSDAEL, J .
EMBO JOURNAL, 1990, 9 (10) :3137-3144
[5]   IDENTIFICATION OF P40X-RESPONSIVE REGULATORY SEQUENCES WITHIN THE HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I LONG TERMINAL REPEAT [J].
BRADY, J ;
JEANG, KT ;
DUVALL, J ;
KHOURY, G .
JOURNAL OF VIROLOGY, 1987, 61 (07) :2175-2181
[6]   REGULATION OF THE HUMAN INTERLEUKIN-2 RECEPTOR ALPHA-CHAIN PROMOTER - ACTIVATION OF A NONFUNCTIONAL PROMOTER BY THE TRANSACTIVATOR GENE OF HTLV-1 [J].
CROSS, SL ;
FEINBERG, MB ;
WOLF, JB ;
HOLBROOK, NJ ;
WONGSTAAL, F ;
LEONARD, WJ .
CELL, 1987, 49 (01) :47-56
[7]   THE PX PROTEIN OF HTLV-I IS A TRANSCRIPTIONAL ACTIVATOR OF ITS LONG TERMINAL REPEATS [J].
FELBER, BK ;
PASKALIS, H ;
KLEINMANEWING, C ;
WONGSTAAL, F ;
PAVLAKIS, GN .
SCIENCE, 1985, 229 (4714) :675-679
[8]   C-FOS PROMOTER TRANS-ACTIVATION BY THE TAX1 PROTEIN OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I [J].
FUJII, M ;
SASSONECORSI, P ;
VERMA, IM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (22) :8526-8530
[9]  
FUJII M, 1991, ONCOGENE, V6, P1023
[10]   INTERACTION OF HTLV-1 TAX1 WITH P67(SRF) CAUSES THE ABERRANT INDUCTION OF CELLULAR IMMEDIATE EARLY GENES THROUGH CARG BOXES [J].
FUJII, M ;
TSUCHIYA, H ;
CHUHJO, T ;
AKIZAWA, T ;
SEIKI, M .
GENES & DEVELOPMENT, 1992, 6 (11) :2066-2076