RAPID QUANTITATION OF INTERFERON WITH CHRONICALLY ONCORNAVIRUS-PRODUCING CELLS

被引：43

作者：

ABOUD, M ^{[1
]}

WEISS, O ^{[1
]}

SALZBERG, S ^{[1
]}

机构：

[1] BAR ILAN UNIV, DEPT LIFE SCI, RAMAT GAN, ISRAEL

来源：

INFECTION AND IMMUNITY | 1976年 / 13卷 / 06期

关键词：

D O I：

10.1128/IAI.13.6.1626-1632.1976

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The capacity of interferon to inhibit virus production in [mouse embryo 3T3] cells chronically infected with [Moloney murine leukemia] oncornavirus enabled development of a simple system for interferon quantitation that was independent of exogenous viral infection. The release of the virus to the culture medium was determined by its reverse transcriptase activity. The inhibitory effect of interferon in this system was linearly proportional to the log of its dilution over a range of 5-80% inhibition. The sensitivity of the system was comparable to that of the vesicular stomatitis virus plaque reduction assay; its reproducibility was even better. This method is very rapid and can be completed within < 24 h.

引用

页码：1626 / 1632

页数：7