DIAGNOSTIC-VALUE OF THE MOLECULAR-GENETIC DETECTION OF THE T(1122) TRANSLOCATION IN EWINGS TUMORS

被引:51
作者
DOCKHORNDWORNICZAK, B
SCHAFER, KL
DANTCHEVA, R
BLASIUS, S
WINKELMANN, W
STREHL, S
BURDACH, S
VANVALEN, F
JURGENS, H
BOCKER, W
机构
[1] UNIV DUSSELDORF,DEPT PEDIAT HEMATOL & ONCOL,W-4000 DUSSELDORF,GERMANY
[2] ST ANNA CHILDRENS HOSP,CHILDRENS CANC RES INST,VIENNA,AUSTRIA
[3] UNIV MUNSTER,DEPT PEDIAT HEMATOL & ONCOL,W-4400 MUNSTER,GERMANY
[4] UNIV MUNSTER,DEPT ORTHOPED SURG,W-4400 MUNSTER,GERMANY
来源
VIRCHOWS ARCHIV-AN INTERNATIONAL JOURNAL OF PATHOLOGY | 1994年 / 425卷 / 02期
关键词
EWINGS TUMOR; TRANSLOCATION; REVERSE TRANSCRIPTION; POLYMERASE CHAIN REACTION;
D O I
10.1007/BF00230345
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
One consistent feature of the Ewing's tumour family is the presence of a balanced translocation involving band q12 and band q24 of chromosome 22 and chromosome 11. Recent cloning of the chromosome breakpoint regions of t(11;22)(q24;q12) Ewing's sarcoma translocation has revealed that the breakpoints were localized within the Ewing's sarcoma gene (EWS gene) on chromosome 22 and the Fli-1 gene on chromosome 11. Molecular genetic techniques can thus be applied to the detection of the t(11;22) translocation in Ewing's tumours. By reverse transcription and polymerase chain reaction technique (RT-PCR) 11 Ewing's tumour derived cell lines, 12 primary Ewing's tumours, and 11 tumours after treatment were analysed for the occurence of the t(11;22) translocation. Furthermore, blood and bone marrow samples from 5 patients were available for RT-PCR. In 78% of the cell lines and 91% of the primary Ewing's tumours the t(11;22) translocation was detectable by RT-PCR. In bone marrow samples from a Ewing's sarcoma patient presenting in relapse tumour cells were detected by molecular genetic analysis. Our results indicate that molecular genetic detection of the t(11;22) translocation is valuable in the differential diagnosis of small round cell rumours and will provide important information for the staging and prognosis of Ewing's tumour.
引用
收藏
页码:107 / 112
页数:6
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