TRANSGALACTOSYLATION CATALYZED BY ALPHA-GALACTOSIDASE FROM CANDIDA-GUILLIERMONDII H-404

被引：26

作者：

HASHIMOTO, H

KATAYAMA, C

GOTO, M

OKINAGA, T

KITAHATA, S

机构：

[1] OSAKA MUNICIPAL TECH RES INST, JOTO KU, OSAKA 536, JAPAN

[2] SUGIYAMA CHEM & IND LAB, TOTSUKA KU, YOKOHAMA, KANAGAWA 245, JAPAN

[3] HONEN CORP, TOKYO 100, JAPAN

[4] TOKYO INST TECHNOL, FAC BIOSCI & BIOTECHNOL, DEPT LIFE SCI, YOKOHAMA, KANAGAWA 227, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1995年 / 59卷 / 04期

关键词：

D O I：

10.1271/bbb.59.619

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The thermostable alpha-galactosidase from Candida guilliermondii H-404 synthesized self-transfer products in the absence of a suitable acceptor. The main self-transfer product, using melibiose as a donor substrate, was O-alpha-D-galactosyl-(1,6)-O-alpha-D-galactosyl-(1,6)-D-glucose. This enzyme had a wide acceptor specificity, D-Glucose, D-galactose, maltose, maltitol, and 1,4-butandiol were the most effective accepters in the transgalactosylation catalyzed by this enzyme, The enzyme could also transfer a-galactosyl residues to pentoses (L-arabinose, D-xylose, and D-ribose) and methyl pentoses (D-fucose and L-rhamnose), The main transfer products to lactose, maltose, and sucrose as accepters were identified as O-alpha-D-galactosyl-(1,6)-O-beta-D-galactosyl-(1,4)-D-glucose, O-alpha-D-galactosyl-(1,6)-O-alpha-D-glucosyl-(1,4)-D-glucose, and O-alpha-D-galactosyl-(1,6)-O-alpha-D-glucosyl-(1,2)-beta-D-fructoside (raffinose), respectively.

引用

页码：619 / 623

页数：5

共 15 条

[1] GALACTOSE AT THE NONREDUCING TERMINUS OF O-LINKED OLIGOSACCHARIDES OF MOUSE EGG ZONA PELLUCIDA GLYCOPROTEIN ZP3 IS ESSENTIAL FOR THE GLYCOPROTEINS SPERM RECEPTOR ACTIVITY [J].