IDENTIFICATION OF SOMATOGENIC BINDING-SITES IN LIVER-MICROSOMES FROM NORMAL MICE AND TRANSGENIC MICE EXPRESSING HUMAN GROWTH-HORMONE GENE

被引：10

作者：

AGUILAR, RC ^{[1
]}

FERNANDEZ, HN ^{[1
]}

DELLACHA, JM ^{[1
]}

CALANDRA, RS ^{[1
]}

BARTKE, A ^{[1
]}

TURYN, D ^{[1
]}

机构：

[1] SO ILLINOIS UNIV,SCH MED,DEPT PHYSIOL,CARBONDALE,IL 62901

来源：

LIFE SCIENCES | 1992年 / 50卷 / 09期

关键词：

D O I：

10.1016/0024-3205(92)90247-M

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Somatogenic binding sites were detected and characterized in microsomal preparations from livers of normal mice and mice expressing metallothionein-I/hGH (mMT/hGH) hybrid gene, using I-125-labeled bovine or human GH, or a photoreactive derivative of hGH (I-125-AP-hGH1). Specific binding of I-125-bGH was detected in liver microsomes from both normal and transgenic mice with an apparent Kd of 2 nM. I-125-hGH was partially displaced by bGH. I-125-AP-hGH1 was covalently bound to the microsomal preparations, and bGH prevented the formation of the 130 kDa species with no appreciable effect on 63 kDa and 70 kDa lactogenic complexes.

引用

页码：615 / 620

页数：6

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