CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC CHARACTERIZATION OF THE COPPER-CONTAINING AMINE OXIDASE FROM PEA-SEEDLINGS

被引：23

作者：

VIGNEVICH, V

DOOLEY, DM

GUSS, JM

HARVEY, I

MCGUIRL, MA

FREEMAN, HC

机构：

[1] UNIV SYDNEY,DEPT INORGAN CHEM,SYDNEY,NSW 2006,AUSTRALIA

[2] AMHERST COLL,DEPT CHEM,AMHERST,MA 01002

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1993年 / 229卷 / 01期

关键词：

AMINE OXIDASE; COPPER PROTEIN; CRYSTALLIZATION; CRYSTAL DENSITY; SPACE GROUP;

D O I：

10.1006/jmbi.1993.1022

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The copper-containing amine oxidase from pea seedlings has been crystallized using lithium sulfate as precipitant at pH 5.2. The unit cell is orthorhombic, space group P212121, with dimensions a = 89.3 AÅ, b = 113.4 AÅ, c = 199.0 AÅ. The mass of the asymmetric unit is 131(±13) kDa, consistent with independent evidence that the molecule has two ˜66 kDa subunits. The crystals diffract to 2.5 AÅ in a synchrotron X-ray beam. © 1993 Academic Press, Inc.

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页码：243 / 245

页数：3

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