A PCR METHOD FOR THE SEQUENCE-ANALYSIS OF THE GYRA, POLA AND RNHA GENE SEGMENTS FROM MYCOBACTERIA

被引：15

作者：

MIZRAHI, V ^{[1
]}

HUBERTS, P ^{[1
]}

DAWES, SS ^{[1
]}

DUDDING, LR ^{[1
]}

机构：

[1] UNIV WITWATERSRAND,SCH MED,DEPT HAEMATOL,JOHANNESBURG 2193,SOUTH AFRICA

来源：

GENE | 1993年 / 136卷 / 1-2期

关键词：

DEGENERATE OLIGODEOXYRIBONUCLEOTIDE PRIMERS; DNA REPLICATION;

D O I：

10.1016/0378-1119(93)90481-H

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Internal segments of the gyrA and polA genes involved in DNA replication of Mycobacterium tuberculosis and rnhA of M. smegmatis, have been amplified by the polymerase chain reaction (PCR) using degenerate oligodeoxyribonucleotide primers based on conserved sequences. The deduced amino acid sequences were 54-66% homologous to the corresponding segments of their Escherichia coli counterparts. This method provides a useful means of cloning genes encoding DNA replication enzymes of mycobacteria.

引用

页码：287 / 290

页数：4