CA2+ HANDLING AND MYOFIBRILLAR CA2+ SENSITIVITY IN FERRET CARDIAC MYOCYTES WITH PRESSURE-OVERLOAD HYPERTROPHY

Experiments were performed in aequorin-loaded left ventricular myocytes isolated from hypertrophied hearts and age-matched controls. Five to six months after postvalvular aortic banding, left ventricular hypertrophy was present, as indicated by a 97% (P < 0.001) increase in the left ventricular weight-to-body weight ratio and a 24% (P < 0.001) increase in cell width. In comparison with controls, the hypertrophied myocytes demonstrated that 1) contraction duration was prolonged by 37% (P < 0.001) and was associated with a 44% (P < 0.001) prolongation of the intracellular Ca2+ transient; 2) peak systolic shortening was decreased by 31% (P < 0.001) and was associated with a 21% (P < 0.001) decrease in peak systolic intracellular Ca2+ concentration; 3) both the peak systolic intracellular Ca2+ concentration-to-peak shortening relationship and the intracellular Ca2+ concentration-to-cell shortening relationship at the time of the peak twitch were shifted downward, suggesting a decrease in myofilament Ca2+ responsiveness; and 4) isoproterenol (5 x 10(-8) M) produced equal increases in the peak systolic intracellular Ca2+ of control and hypertrophied myocytes (88 vs. 90%; P > 0.05) in contrast to much smaller increases in the peak cell shortening (170 vs. 73%; P < 0.02) of the hypertrophied myocytes, suggesting a decrease in myofilament Ca2+ responsiveness. These data demonstrate that the hypertrophy-related abnormalities in intracellular Ca2+ handling and mechanical function, previously reported in aequorin-loaded multicellular muscle preparations, are present in isolated myocytes, arguing against changes in the interstitium as essential causative factors.