MURINE POLYOMAVIRUS AND SIMIAN-VIRUS-40 LARGE T-ANTIGENS PRODUCE DIFFERENT STRUCTURAL ALTERATIONS IN VIRAL ORIGIN DNA

被引:14
作者
BHATTACHARYYA, S [1 ]
LORIMER, HE [1 ]
PRIVES, C [1 ]
机构
[1] COLUMBIA UNIV,DEPT BIOL SCI,NEW YORK,NY 10027
关键词
D O I
10.1128/JVI.69.12.7579-7585.1995
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Murine polyomavirus (Py) and simian virus 40 (SV40) encode homologous large T antigens (T Ags) and also have comparable sequence motifs in their core replication origins. While the ability of SV40 T Ag to produce specific distortions,within the SV40 core replication origin (ori) in a nucleotide-dependent fashion has been well documented, little is known about related effects of Py T Ag on Py ori DNA. Therefore, we have examined viral origin DNA binding in the presence of nucleotide and the resulting structural changes induced by Py and SV40 T Ags by DNase I footprinting and KMnO4 modification assays. The structural changes in the Py ori induced by Py T Ag included sites within both the AIT and early side of the core origin region, consistent with what has been shown for SV40. Interestingly, however, Py T Ag also produced sites of distortion within the center of the origin palindrome and at several sites within both the early and late regions that flank the core ori. Thus, Py T Ag produces a more extensive and substantially different pattern of KMnO4, modification sites than does SV40 T Ag. We also observed that both T Ags incompletely protected and distorted the reciprocal ori region. Therefore, significant differences in the interactions of Py and SV40 T Ags with ori DNA may account for the failure of each T Ag to support replication of the reciprocal ori DNA in permissive cell extracts.
引用
收藏
页码:7579 / 7585
页数:7
相关论文
共 64 条
[31]   SEQUENCES FLANKING THE PENTANUCLEOTIDE T-ANTIGEN BINDING-SITES IN THE POLYOMAVIRUS CORE ORIGIN HELP DETERMINE SELECTIVITY OF DNA-REPLICATION [J].
LI, L ;
LI, BL ;
HOCK, M ;
WANG, E ;
FOLK, WR .
JOURNAL OF VIROLOGY, 1995, 69 (12) :7570-7578
[32]   THE DNA-BINDING PROPERTIES OF POLYOMAVIRUS LARGE T-ANTIGEN ARE ALTERED BY ATP AND OTHER NUCLEOTIDES [J].
LORIMER, HE ;
WANG, EH ;
PRIVES, C .
JOURNAL OF VIROLOGY, 1991, 65 (02) :687-699
[33]   INHIBITION OF POLYOMA DNA-SYNTHESIS BY BASE PAIR SUBSTITUTIONS AT THE REPLICATION ORIGIN [J].
LUTHMAN, H ;
OSTERLUND, M ;
MAGNUSSON, G .
NUCLEIC ACIDS RESEARCH, 1984, 12 (19) :7503-7515
[34]   NON-CONTIGUOUS SEGMENTS OF THE POLYOMA GENOME REQUIRED IN CIS FOR DNA-REPLICATION [J].
LUTHMAN, H ;
NILSSON, MG ;
MAGNUSSON, G .
JOURNAL OF MOLECULAR BIOLOGY, 1982, 161 (04) :533-550
[35]   IDENTIFICATION OF CRITICAL ELEMENTS WITHIN THE JC VIRUS-DNA REPLICATION ORIGIN [J].
LYNCH, KJ ;
FRISQUE, RJ .
JOURNAL OF VIROLOGY, 1990, 64 (12) :5812-5822
[36]   HUMAN-EMBRYONIC KIDNEY-CELLS - STABLE TRANSFORMATION WITH AN ORIGIN-DEFECTIVE SIMIAN VIRUS-40 DNA AND USE AS HOSTS FOR HUMAN PAPOVAVIRUS REPLICATION [J].
MAJOR, EO ;
MATSUMURA, P .
MOLECULAR AND CELLULAR BIOLOGY, 1984, 4 (02) :379-382
[37]   ATP-DEPENDENT ASSEMBLY OF DOUBLE HEXAMERS OF SV40 T-ANTIGEN AT THE VIRAL ORIGIN OF DNA-REPLICATION [J].
MASTRANGELO, IA ;
HOUGH, PVC ;
WALL, JS ;
DODSON, M ;
DEAN, FB ;
HURWITZ, J .
NATURE, 1989, 338 (6217) :658-662
[38]   A UNIQUE SUBPOPULATION OF MURINE DNA-POLYMERASE ALPHA/PRIMASE SPECIFICALLY INTERACTS WITH POLYOMAVIRUS T-ANTIGEN AND STIMULATES DNA-REPLICATION [J].
MOSES, K ;
PRIVES, C .
MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (04) :2767-2776
[39]   POLYOMAVIRUS ORIGIN FOR DNA-REPLICATION COMPRISES MULTIPLE GENETIC ELEMENTS [J].
MULLER, WJ ;
MUELLER, CR ;
MES, AM ;
HASSELL, JA .
JOURNAL OF VIROLOGY, 1983, 47 (03) :586-599
[40]   ROLE OF DNA POLYMERASE-ALPHA AND DNA PRIMASE IN SIMIAN VIRUS-40 DNA-REPLICATION INVITRO [J].
MURAKAMI, Y ;
WOBBE, CR ;
WEISSBACH, L ;
DEAN, FB ;
HURWITZ, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (09) :2869-2873