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MURINE POLYOMAVIRUS AND SIMIAN-VIRUS-40 LARGE T-ANTIGENS PRODUCE DIFFERENT STRUCTURAL ALTERATIONS IN VIRAL ORIGIN DNA
被引:14
作者:
BHATTACHARYYA, S
[1
]
LORIMER, HE
[1
]
PRIVES, C
[1
]
机构:
[1] COLUMBIA UNIV,DEPT BIOL SCI,NEW YORK,NY 10027
关键词:
D O I:
10.1128/JVI.69.12.7579-7585.1995
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
Murine polyomavirus (Py) and simian virus 40 (SV40) encode homologous large T antigens (T Ags) and also have comparable sequence motifs in their core replication origins. While the ability of SV40 T Ag to produce specific distortions,within the SV40 core replication origin (ori) in a nucleotide-dependent fashion has been well documented, little is known about related effects of Py T Ag on Py ori DNA. Therefore, we have examined viral origin DNA binding in the presence of nucleotide and the resulting structural changes induced by Py and SV40 T Ags by DNase I footprinting and KMnO4 modification assays. The structural changes in the Py ori induced by Py T Ag included sites within both the AIT and early side of the core origin region, consistent with what has been shown for SV40. Interestingly, however, Py T Ag also produced sites of distortion within the center of the origin palindrome and at several sites within both the early and late regions that flank the core ori. Thus, Py T Ag produces a more extensive and substantially different pattern of KMnO4, modification sites than does SV40 T Ag. We also observed that both T Ags incompletely protected and distorted the reciprocal ori region. Therefore, significant differences in the interactions of Py and SV40 T Ags with ori DNA may account for the failure of each T Ag to support replication of the reciprocal ori DNA in permissive cell extracts.
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页码:7579 / 7585
页数:7
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