SAMPLING FLUID FROM SLICE CHAMBERS BY MICROSIPHONING

被引:14
作者
GODFREY, DA
WALLER, HJ
机构
[1] MED COLL OHIO,DEPT ANAT,TOLEDO,OH 43699
[2] MED COLL OHIO,DEPT PHYSIOL & BIOPHYS,TOLEDO,OH 43699
[3] MED COLL OHIO,DEPT NEUROL SURG,TOLEDO,OH 43699
关键词
BRAIN SLICE; COCHLEAR NUCLEUS; AMINO ACIDS; RELEASE; MICROSIPHONING; NEURONAL FIRING; EFFLUENT SAMPLING; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY;
D O I
10.1016/0165-0270(92)90058-L
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We have developed a simple technique that continuously samples perfusion fluid from an in vitro slice chamber and permits simultaneous electrophysiological recordings from a slice. This procedure uses a microsiphon that diverts approximately 1.5% of the total effluent of artificial cerebrospinal fluid from an interface slice chamber into a collection tube at a lower level outside the chamber. The tip of the microsiphon may be moved anywhere within the chamber without disturbing the slice. For optimum sampling of the release of substances from one area of a slice, the tip is positioned immediately downstream from the location being studied. Concentrations of several amino acids were measured to identify significant spatial and temporal characteristics of the technique and to demonstrate that spontaneous and induced release of amino acids from slices can be measured with adequate sensitivity and time resolution.
引用
收藏
页码:167 / 173
页数:7
相关论文
共 8 条
[1]   INVITRO MICRODIALYSIS - A NOVEL TECHNIQUE FOR STIMULATED NEUROTRANSMITTER RELEASE MEASUREMENTS [J].
BRADBERRY, CW ;
SPROUSE, JS ;
SHELDON, PW ;
AGHAJANIAN, GK ;
ROTH, RH .
JOURNAL OF NEUROSCIENCE METHODS, 1991, 36 (01) :85-90
[2]  
Freeze A, 1979, GROUNDWATER
[3]   SIMPLE PERFUSION CHAMBER FOR THE STUDY OF NERVOUS-TISSUE SLICES INVITRO [J].
HAAS, HL ;
SCHAERER, B ;
VOSMANSKY, M .
JOURNAL OF NEUROSCIENCE METHODS, 1979, 1 (04) :323-325
[4]   HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF AMINO-ACIDS IN THE PICOMOLE RANGE [J].
HILL, DW ;
WALTERS, FH ;
WILSON, TD ;
STUART, JD .
ANALYTICAL CHEMISTRY, 1979, 51 (08) :1338-1341
[5]   A NEW METHOD FOR THE MEASUREMENT OF ENDOGENOUS TRANSMITTER RELEASE IN LOCALIZED REGIONS OF HIPPOCAMPAL SLICES [J].
ROISIN, MP ;
BRASSART, JL ;
CHARTON, G ;
CREPEL, V ;
BENARI, Y .
JOURNAL OF NEUROSCIENCE METHODS, 1991, 37 (02) :183-189
[6]  
ROSS CD, 1989, VISION RES, V29, P1079
[7]   RAPID CHEMICAL-SAMPLING OF ENDOGENOUS SPECIES FROM BRAIN SLICE PREPARATIONS [J].
SHIEKHATTAR, R ;
BIBBS, EG ;
SCHOWEN, RL ;
ADAMS, RN .
NEUROSCIENCE LETTERS, 1990, 111 (1-2) :189-194
[8]  
WALLER HJ, 1991, ABST SOC NEUR, V21, P412