MOLECULAR-CLONING OF THE HUMAN BRAIN AND GASTRIC CHOLECYSTOKININ RECEPTOR - STRUCTURE, FUNCTIONAL EXPRESSION AND CHROMOSOMAL LOCALIZATION

被引：216

作者：

PISEGNA, JR

DEWEERTH, A

HUPPI, K

WANK, SA

机构：

[1] NIDDKD, DIGEST DIS BRANCH, BETHESDA, MD 20892 USA

[2] NIH, MOLEC GENET SECT, GENET LAB, BETHESDA, MD 20892 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 189卷 / 01期

关键词：

D O I：

10.1016/0006-291X(92)91557-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The receptors for the brain and gastrointestinal peptide, cholecystokinin, can be classfied into CCKA and CCKB subtypes. Having recently cloned the rat CCKB receptor, we used it's cDNA to isolate the human CCKB receptor homologue from brain and stomach which encodes a 447 amino acid protein with 90% identity to both rat CCKB and canine gastrin receptors. Northern hybridization identifies transcripts from stomach, pancreas, brain and gallbladder. The CCKB receptor gene maps to chromosome 11. Expression of the receptor cDNA in COS-7 cells was characteristics of a CCKB receptor subtype pharmacology. These data confirm that we have cloned a novel gene for the human brain and stomach CCKB receptor. © 1992.

引用

页码：296 / 303

页数：8

共 34 条

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