EFFECT OF CONSTANT AND MODULATED ELECTRICAL CHARGES APPLIED TO THE CULTURE MATERIAL ON PGI2 AND TXA2 SECRETION BY ENDOTHELIAL-CELLS

Electrical stimulation of an endothelial cell culture applied through the culture support biomaterial induced strong secretion of prostaglandin 12 (PG12) and, to a lesser extent, thromboxane A2 (TXA2), which varied with time, the sign of the stationary electrical charge and the amplitude and frequency of a superimposed sine wave. In stationary conditions, the response is more rapid at positive electrical charges (+ some tens of mu C cm(-2)) than at negative ones (- some tens of mu C cm(-2)). In sine wave conditions, the ratio [PG12]/[TXA2] is strongly increased and is maximum for an amplitude of 50 mV and a frequency of 1 Hz. These results suggest that the extracellular matrix is not only involved in cellular anchoring but also participates actively in secretion of thrombomodulatory substances, an effect apparently related to the piezoelectric properties of its components.