CYANIDE DETERMINATION USING AN AMPEROMETRIC BIOSENSOR BASED ON CYTOCHROME-OXIDASE INHIBITION

被引:62
作者
AMINE, A
ALAFANDY, M
KAUFFMANN, JM
PEKLI, MN
机构
[1] FREE UNIV BRUSSELS,INST PHARM CAMPUS PLAINE,B-1050 BRUSSELS,BELGIUM
[2] SEMMELWEIS UNIV MED,DEPT PHARMACEUT CHEM,H-1092 BUDAPEST,HUNGARY
关键词
D O I
10.1021/ac00113a015
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An amperometric reagentless biosensor, based on a carbon paste electrode (CPE) modified by asolectin, cytochrome c, and cytochrome oxidase, is described for the sensitive determination of cyanide. The modified CP matrix mimics a biological membrane environment. The sensor, polarized at -0.15 V vs Ag/Ag/Cl, generates the reduced form of cytochrome c, which in turn is oxidized by the enzyme cytochrome oxidase. The resulting current is related to the enzyme activity and is depressed by inhibitors of cytochrome oxidase such as cyanide. Concentrations of cyanide as low as 0.5 mu M can be measured with half-maximal response at about 12 mu M. The effects of pH, ionic strength, and temperature on this new cyanide biosensor are reported. The inhibition is reversible and reproducible (RSD = 4%), allowing cyanide determination for more than 2 months using the same probe. Possible use of this biosensor in flow systems is illustrated.
引用
收藏
页码:2822 / 2827
页数:6
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