APPLICATION OF LIGAND-EXCHANGE CHROMATOGRAPHY TO THE ASSAY OF L-ALANINE FROM DL-ASPARTIC ACID BY PSEUDOMONAS-DACUNHAE

被引：4

作者：

CHENG, CY

HUANG, IH

机构：

[1] Department of Chemistry, Chung Yuan Christian University, Chung-Li

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 555卷 / 1-2期

关键词：

D O I：

10.1016/S0021-9673(01)87189-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A direct chiral ligand-exchange chromatographic method was developed to monitor L-alanine production by fermentation. A mobile phase containing aqueous 0.25 mM Zn2+ solution is utilized to separate amino acids in the fermentation medium. The detection limit for L-alanine is 0.5 ppm and the analysis time for one sample is about 8 min. As sample preparation is simple and the matrix effects are minimal, the assay is fast and convenient. The results indicate that the method has potential for the analysis of complicated fermentation media.

引用

页码：272 / 277

页数：6

共 14 条

[11] INDUSTRIAL-PRODUCTION OF D-ASPARTIC ACID AND L-ALANINE FROM DL-ASPARTIC ACID USING A PRESSURIZED COLUMN REACTOR CONTAINING IMMOBILIZED PSEUDOMONAS-DACUNHAE CELLS [J].