A RAPID AND SENSITIVE MICROASSAY FOR DETERMINATION OF CHITINOLYTIC ACTIVITY

被引：124

作者：

MCCREATH, KJ ^{[1
]}

GOODAY, GW ^{[1
]}

机构：

[1] UNIV ABERDEEN MARISCHAL COLL, DEPT MOLEC & CELL BIOL, ABERDEEN AB9 1AS, SCOTLAND

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1992年 / 14卷 / 04期

关键词：

N-ACETYLGLUCOSAMINIDASE; CHITINASE; FLUOROGENIC SUBSTRATE;

D O I：

10.1016/0167-7012(92)90055-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid and sensitive assay for chitinolytic activity has been developed on a micro-scale. This assay is based on the use of fluorogenic 4-methylumbelliferone glycosides of N-acetylglucosamine oligosaccharides in order to characterise the enzymes associated with chitinolytic microorganisms, i.e. exo- and endochitinases and N-acetylglucosamimidase. The sensitivity of the assay allows detailed kinetic studies to be performed. Examples of its uses are given for chitinolytic activities from an estuarine bacterial isolate.

引用

页码：229 / 237

页数：9

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