PREPARATION AND OPTIMIZATION OF A CELL-FREE TRANSLATION SYSTEM FROM VICIA-SATIVA GERM LACKING RIBOSOME-INACTIVATING PROTEIN-ACTIVITY

被引：24

作者：

ARIAS, FJ ^{[1
]}

ROJO, MA ^{[1
]}

FERRERAS, JM ^{[1
]}

IGLESIAS, R ^{[1
]}

MUNOZ, R ^{[1
]}

GIRBES, T ^{[1
]}

机构：

[1] UNIV VALLADOLID,FAC CIENCIAS,DEPT BIOQUIM BIOL MOLEC & FISIOL,E-47005 VALLADOLID,SPAIN

来源：

JOURNAL OF EXPERIMENTAL BOTANY | 1992年 / 43卷 / 250期

关键词：

TRANSLATION SYSTEM; PROTEIN SYNTHESIS; CELL-FREE EXTRACTS; RIBOSOME-INACTIVATING PROTEINS; VICIA-SATIVA;

D O I：

10.1093/jxb/43.5.729

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A cell-free translation system from Vicia sativa with high activity for reading endogenous messengers was prepared. The concentrations of several of the components were optimized. The system was highly sensitive to cycloheximide, puromycin and fusidic acid, but was completely insensitive to chloramphenicol. The system lacks apparent free endogenous ribosome-inactivating protein activity. It was highly sensitive to several well-known ribosome-inactivating proteins, which makes it very suitable for studying the effects and the mechanism of action of these plant toxins.

引用

页码：729 / 737

页数：9

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