INTERACTION OF RNA POLYMERASE-I TRANSCRIPTION FACTORS WITH A PROMOTER IN THE NONTRANSCRIBED SPACER OF RAT RIBOSOMAL DNA

被引：21

作者：

SMITH, SD ^{[1
]}

ORIAHI, E ^{[1
]}

YANGYEN, HF ^{[1
]}

XIE, WQ ^{[1
]}

CHEN, C ^{[1
]}

ROTHBLUM, LI ^{[1
]}

机构：

[1] WEIS CTR RES,GEISINGER CLIN,DANVILLE,PA 17822

来源：

NUCLEIC ACIDS RESEARCH | 1990年 / 18卷 / 07期

关键词：

D O I：

10.1093/nar/18.7.1677

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The spacer promoter of the rat rDNA repeat consists of two functional domains: a core (proximal) element that is sufficient for transcription In vitro, and an upstream (distal) promoter element that increases the efficiency of transcription. Two of the transcription factors that interact with the 45S promoter also interact with the spacer promoter. Rat SL-1, is required for transcription of the spacer promoter by heterologous extracts, e.g. human, and rat SF-1 is required for efficient transcription in vitro. Order-of-addition experiments demonstrated that the preinitiation complex formed by these factors on the spacer promotor Is not as stable as the complex formed on the 45S promoter. DNase 1 footprlnting experiments demonstrated binding sites for rat SL-1 and SF-1 on the distal element of the spacer promoter. The topology of the domains of the spacer promoter may explain both the reduced stability of the preinitiation complex . formed on that promoter and the lower efficiency of transcription of that promo ter when compared to the 45S promoter. © 1990 Oxford University Press.

引用

页码：1677 / 1685

页数：9

共 51 条

[41] STRUCTURAL DETERMINANT OF THE SPECIES-SPECIFIC TRANSCRIPTION OF THE MOUSE RIBOSOMAL-RNA GENE PROMOTER [J].