VERAPAMIL ENHANCES HIGH-DENSITY-LIPOPROTEIN PROCESSING IN HEP G2 CELLS PRELOADED WITH CHOLESTEROL

被引：1

作者：

CHAPPEYGILLET, B ^{[1
]}

SALMON, S ^{[1
]}

MAZIERE, C ^{[1
]}

AUCLAIR, M ^{[1
]}

MAZIERE, JC ^{[1
]}

机构：

[1] UNIV PARIS 06,BIOCHIM LAB,27 RUE CHALIGNY,F-75571 PARIS 12,FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1052卷 / 02期

关键词：

(Human hepatocyte); Cholesterol; HDL; Verapamil;

D O I：

10.1016/0167-4889(90)90221-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of the calcium channel blocker of the arylalkylamine series verapamil have been investigated on high-density lipoprotein (HDL3) catabolism in the human hepatoma cell line Hep G2. It was found that verapamil markedly enhanced HDL3 binding, uptake and degradation in Hep G2 cells preloaded with nonlipoprotein cholesterol. This effect was dose-dependent, and a 1.5-2-fold increase of the three studied parameters was observed in cells pretreated 24 h with 100 μM verapamil. No significant effect of the drug was found in cells not preincubated with cholesterol. Verapamil induced an increase in the cellular cholesterol content in preloaded cells. Other calcium antagonists such as diltiazem, nifedipine, nitrendipine or amphiphilic drugs such as phenothiazines and propranolol also enhanced HDL3 uptake by Hep G2 cells. These effects of verapamil on HDL3 metabolism could be related to its amphiphilic characteristics, and to its calcium antagonist properties. © 1990.

引用

页码：273 / 277

页数：5

共 24 条

[1] METABOLISM OF VERY LOW-DENSITY LIPOPROTEIN PROTEINS .1. PRELIMINARY IN-VITRO AND IN-VIVO OBSERVATIONS [J].

BILHEIMER, DW ;

LEVY, RI ;

EISENBERG, S .

BIOCHIMICA ET BIOPHYSICA ACTA, 1972, 260 (02) :212-+

[2] THE EFFECTS OF PERHEXILINE-MALEATE ON LOW-DENSITY LIPOPROTEIN PROCESSING AND CHOLESTEROL-METABOLISM IN CULTURED HUMAN-FIBROBLASTS [J].

CANDIDE, C ;

MAZIERE, JC ;

MAZIERE, C ;

LAGERON, A ;

POLONOVSKI, J .

EUROPEAN JOURNAL OF CLINICAL PHARMACOLOGY, 1988, 34 (02) :195-199

[3]

CORSINI A, 1986, PHARMACOL RES, V18, P1

[4] BINDING AND DEGRADATION OF HUMAN HIGH-DENSITY LIPOPROTEINS BY HUMAN HEPATOMA-CELL LINE HEPG2 [J].

DASHTI, N ;

WOLFBAUER, G ;

ALAUPOVIC, P .

BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 833 (01) :100-110

[5] CALCIUM-CHANNEL BLOCKERS STIMULATE LDL RECEPTOR SYNTHESIS IN HUMAN-SKIN FIBROBLASTS [J].

FILIPOVIC, I ;

BUDDECKE, E .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1986, 136 (03) :845-850

[6] INTERACTIONS OF A PHENOTHIAZINE TRANQUILIZER WITH PHOSPHATIDYLCHOLINE AND PHOSPHATIDYLCHOLINE CHOLESTEROL MEMBRANES [J].

FORREST, BJ ;

LINEHAN, PTP ;

MATTAI, J .

BIOCHEMISTRY, 1984, 23 (10) :2288-2293

[7] EFFECT OF L-PROPRANOLOL ON THE BINDING, INTERNALIZATION AND DEGRADATION OF I-125 LOW-DENSITY LIPOPROTEINS BY HUMAN-SKIN FIBROBLASTS [J].

GHISELLI, G ;

BERNINI, F ;

MUSANTI, R ;

FUMAGALLI, R .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1981, 103 (03) :1060-1068

[8]

GOLSTEIN JL, 1977, METABOLISM, V26, P1257

[9] DISTRIBUTION AND CHEMICAL COMPOSITION OF ULTRACENTRIFUGALLY SEPARATED LIPOPROTEINS IN HUMAN SERUM [J].

HAVEL, RJ ;

EDER, HA ;

BRAGDON, JH .

JOURNAL OF CLINICAL INVESTIGATION, 1955, 34 (09) :1345-1353

[10]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

← 1 2 3 →