MESSENGER-RNA LEVELS AND METHYLATION PATTERNS OF GAPDH AND BETA-ACTIN GENES IN RAT-LIVER, SPLEEN AND BRAIN IN RELATION TO AGING

被引：59

作者：

SLAGBOOM, PE ^{[1
]}

DELEEUW, WJF ^{[1
]}

VIJG, J ^{[1
]}

机构：

[1] TNO,INST EXPTL GERONTOL,DEPT MOLEC BIOL,POB 5815,2280 HV RIJSWIJK,NETHERLANDS

来源：

MECHANISMS OF AGEING AND DEVELOPMENT | 1990年 / 53卷 / 03期

关键词：

Aging; DNA methylation; GAPDH; Gene expression; mRNA; β-Actin;

D O I：

10.1016/0047-6374(90)90042-E

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Messenger RNA levels and methylation patterns of the glyceraldehyde-3-phosphate-dehydrogenase (GAPHD) and β-actin genes were studied in spleen, liver and brain of 6-, 24- and 36-month old female inbred rats. In the spleen, the mRNA levels of both housekeeping genes significantly increased between 24 and 36 months. No age-related alterations in the expression of GAPDH or β-actin mRNA were observed in brain or liver. A considerable intertissue and interindividual variation was observed in the mRNA levels of these genes in all age-groups as compared to the level of 28 S rRNA, which was used as an internal control. In this respect the interindividual variation in the level of GAPDH mRNA paralleled the variation observed in the β-actin mRNA level in the three tissues studied. The methylation pattern of β-actin was found to be tissue-specific in contrast to that of GAPDH, which was identical in all three tissues. No significant age-related alterations were observed in the GAPDH methylation pattern, whereas β-actin appeared to become slightly demethylated with age in the spleen at the CpG site for which tissue-specificity was observed. © 1990.

引用

页码：243 / 257

页数：15

共 29 条

[1]

AUFFRAY C, 1980, EUR J BIOCHEM, V107, P303

[2] DNA METHYLATION - HOW IMPORTANT IN GENE-CONTROL [J].

BIRD, AP .

NATURE, 1984, 307 (5951) :503-504

[3] DNA METHYLATION AND GENE ACTIVITY [J].

CEDAR, H .

CELL, 1988, 53 (01) :3-4

[4]

CHATTERJEE B, 1981, J BIOL CHEM, V256, P5939

[5] GENOMIC SEQUENCING [J].

CHURCH, GM ;

GILBERT, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (07) :1991-1995

[6] QUANTITATIVE COMPARISON OF MESSENGER-RNA LEVELS IN MAMMALIAN-TISSUES - 28S RIBOSOMAL-RNA LEVEL AS AN ACCURATE INTERNAL CONTROL [J].

DELEEUW, WJF ;

SLAGBOOM, PE ;

VIJG, J .

NUCLEIC ACIDS RESEARCH, 1989, 17 (23) :10137-10138

[7] THE GENES-CODING FOR THE CYTOSKELETAL PROTEINS ACTIN AND VIMENTIN IN WARM-BLOODED VERTEBRATES [J].

DODEMONT, HJ ;

SORIANO, P ;

QUAX, WJ ;

RAMAEKERS, F ;

LENSTRA, JA ;

GROENEN, MAM ;

BERNARDI, G ;

BLOEMENDAL, H .

EMBO JOURNAL, 1982, 1 (02) :167-171

[8] HUMAN-LYMPHOCYTES AGED INVIVO HAVE REDUCED LEVELS OF METHYLATION IN TRANSCRIPTIONALLY ACTIVE AND INACTIVE DNA [J].

DRINKWATER, RD ;

BLAKE, TJ ;

MORLEY, AA ;

TURNER, DR .

MUTATION RESEARCH, 1989, 219 (01) :29-37

[9] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[10] VARIOUS RAT ADULT TISSUES EXPRESS ONLY ONE MAJOR MESSENGER-RNA SPECIES FROM THE GLYCERALDEHYDE-3-PHOSPHATE-DEHYDROGENASE MULTIGENIC FAMILY [J].

FORT, P ;

MARTY, L ;

PIECHACZYK, M ;

ELSABROUTY, S ;

DANI, C ;

JEANTEUR, P ;

BLANCHARD, JM .

NUCLEIC ACIDS RESEARCH, 1985, 13 (05) :1431-1442

← 1 2 3 →