MESSENGER-RNA LEVELS AND METHYLATION PATTERNS OF GAPDH AND BETA-ACTIN GENES IN RAT-LIVER, SPLEEN AND BRAIN IN RELATION TO AGING

被引：59

作者：

SLAGBOOM, PE ^{[1
]}

DELEEUW, WJF ^{[1
]}

VIJG, J ^{[1
]}

机构：

[1] TNO,INST EXPTL GERONTOL,DEPT MOLEC BIOL,POB 5815,2280 HV RIJSWIJK,NETHERLANDS

来源：

MECHANISMS OF AGEING AND DEVELOPMENT | 1990年 / 53卷 / 03期

关键词：

Aging; DNA methylation; GAPDH; Gene expression; mRNA; β-Actin;

D O I：

10.1016/0047-6374(90)90042-E

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Messenger RNA levels and methylation patterns of the glyceraldehyde-3-phosphate-dehydrogenase (GAPHD) and β-actin genes were studied in spleen, liver and brain of 6-, 24- and 36-month old female inbred rats. In the spleen, the mRNA levels of both housekeeping genes significantly increased between 24 and 36 months. No age-related alterations in the expression of GAPDH or β-actin mRNA were observed in brain or liver. A considerable intertissue and interindividual variation was observed in the mRNA levels of these genes in all age-groups as compared to the level of 28 S rRNA, which was used as an internal control. In this respect the interindividual variation in the level of GAPDH mRNA paralleled the variation observed in the β-actin mRNA level in the three tissues studied. The methylation pattern of β-actin was found to be tissue-specific in contrast to that of GAPDH, which was identical in all three tissues. No significant age-related alterations were observed in the GAPDH methylation pattern, whereas β-actin appeared to become slightly demethylated with age in the spleen at the CpG site for which tissue-specificity was observed. © 1990.

引用

页码：243 / 257

页数：15

共 29 条

[11]

GERSHON D, 1985, INTERRELATIONSHIP AG, P143

[12] METHYLATION PATTERNS IN THE GENE FOR THE ALPHA-SUBUNIT OF CHORIONIC-GONADOTROPIN ARE INHERITED WITH VARIABLE FIDELITY IN CLONAL LINEAGES OF HUMAN-FIBROBLASTS [J].

GOLDSTEIN, S ;

REIS, RJS .

NUCLEIC ACIDS RESEARCH, 1985, 13 (19) :7055-7065

[13] THE INHERITANCE OF EPIGENETIC DEFECTS [J].

HOLLIDAY, R .

SCIENCE, 1987, 238 (4824) :163-170

[14]

HORBACH GJM, 1986, TOPICS AGING RES EUR, V7, P121

[15] CONSTRUCTION AND CHARACTERIZATION OF GENOMIC LIBRARIES FROM SPECIFIC HUMAN-CHROMOSOMES [J].

KRUMLAUF, R ;

JEANPIERRE, M ;

YOUNG, BD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (09) :2971-2975

[16] RNA MOLECULAR-WEIGHT DETERMINATIONS BY GEL-ELECTROPHORESIS UNDER DENATURING CONDITIONS, A CRITICAL RE-EXAMINATION [J].

LEHRACH, H ;

DIAMOND, D ;

WOZNEY, JM ;

BOEDTKER, H .

BIOCHEMISTRY, 1977, 16 (21) :4743-4751

[17] AGE-DEPENDENT ACCUMULATION OF ALKALI-LABILE SITES IN DNA OF POST-MITOTIC BUT NOT IN THAT OF MITOTIC RAT-LIVER CELLS [J].

MULLAART, E ;

BOERRIGTER, METI ;

BROUWER, A ;

BERENDS, F ;

VIJG, J .

MECHANISMS OF AGEING AND DEVELOPMENT, 1988, 45 (01) :41-49

[18] THE NUCLEOTIDE-SEQUENCE OF THE RAT CYTOPLASMIC BETA-ACTIN GENE [J].

NUDEL, U ;

ZAKUT, R ;

SHANI, M ;

NEUMAN, S ;

LEVY, Z ;

YAFFE, D .

NUCLEIC ACIDS RESEARCH, 1983, 11 (06) :1759-1771

[19] AGE-ASSOCIATED CHANGES IN DNA METHYLATION AND MESSENGER-RNA LEVEL OF THE C-MYC GENE IN SPLEEN AND LIVER OF MICE [J].

ONO, T ;

TAKAHASHI, N ;

OKADA, S .

MUTATION RESEARCH, 1989, 219 (01) :39-50

[20] POST-TRANSCRIPTIONAL REGULATION OF GLYCERALDEHYDE-3-PHOSPHATE-DEHYDROGENASE GENE-EXPRESSION IN RAT-TISSUES [J].

PIECHACZYK, M ;

BLANCHARD, JM ;

MARTY, L ;

DANI, C ;

PANABIERES, F ;

ELSABOUTY, S ;

FORT, P ;

JEANTEUR, P .

NUCLEIC ACIDS RESEARCH, 1984, 12 (18) :6951-6963

← 1 2 3 →