DETERMINATION OF (2S, 3S, 5R)-3-METHYL-7-OXO-3-(1H-1,2,3-TRIAZOL-1-YLMETHYL)-4-THIA-1-AZABICYCLO[3.2.0]HEPTANE-2-CARBOXYLIC ACID 4,4-DIOXIDE (YTR-830H) AND PIPERACILLIN IN PHARMACEUTICAL PREPARATIONS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A high-performance liquid chromatographic method using a wavelength-scanning system was developed for the determination of (2S, 3S, 5R)-3-methyl-7-oxo-3-(1H-1, 2, 3-triazol-1-ylmethyl)-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid 4,4-dioxide (I) and piperacillin (PIPC) in pharmaceutical preparations. I and PIPC were determined using a reversed-phase column with a mixture of 10 mM tetra-n-butylammonium hydroxide and 5 mM K2SO4 (pH 4.1), acetonitrile and methanol (1000:300:25) as the mobile phase. Addition of SO42- to the mobile phase was useful for the separation of related substances and improving the peak tailing of I and PIPC. This mobile phase was also suitable for the determination of PIPC, I and their degradation products in pharmaceutical preparations. Detection was based on the ultraviolet absorption of I at 220 nm and of PIPC at 270 nm using a wavelength-scanning system. The calibration graphs were linear over the ranges 0-7.5 mu g for I and 0-30 mu g for PIPC. The precisions (relative standard deviations of six analyses) of I and PIPC were 0.45% and 0.33%, respectively.