IMAGING BACTERIAL POLYSACCHARIDES BY AFM

被引：35

作者：

GUNNING, AP

KIRBY, AR

MORRIS, VJ

WELLS, B

BROOKER, BE

机构：

[1] JOHN INNES CTR PLANT SCI RES, DEPT CELL BIOL, NORWICH NR4 7UH, NORFOLK, ENGLAND

[2] READING LAB, FOOD RES INST, READING RG6 2EF, BERKS, ENGLAND

来源：

POLYMER BULLETIN | 1995年 / 34卷 / 5-6期

关键词：

D O I：

10.1007/BF00423359

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Atomic force microscopy has been used to image the bacterial polysaccharides xanthan, acetan and gellan. Images were obtained under constant force conditions in a liquid cell. Drops of dilute solutions of the polysaccharides were deposited onto freshly cleaved surfaces of mica and allowed to dry in air. The deposits were then imaged under butanol. Xanthan and acetan form entangled networks upon deposition. Individual molecules can be identified. Under suitable circumstances it has been possible to image the helical structure of acetan. Gellan forms gels upon concentration during drying and images have been obtained of the gel network.

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页码：615 / 619

页数：5

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