ADVANTAGE OF A RAPID EXTRACTION METHOD OF HIV-1 DNA SUITABLE FOR POLYMERASE CHAIN-REACTION

被引：18

作者：

VIGNOLI, C

DELAMBALLERIE, X

ZANDOTTI, C

TAMALET, C

DEMICCO, P

机构：

[1] Laboratoire de Virologie, Hôpital de la Timone, 13385 Marseille, Bd. Jean Moulin

来源：

RESEARCH IN VIROLOGY | 1995年 / 146卷 / 02期

关键词：

HIV; DNA; PCR; CHELATING RESIN; EXTRACTION; CHELEX-100; METHODOLOGY;

D O I：

10.1016/0923-2516(96)81085-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学]; 100705 [微生物与生化药学];

摘要：

We describe a new protocol for extraction of DNA suitable for HIV1 gene amplification from clinical samples using ''Chelex-100'' chelating resin. Comparison was made with the classic proteinase K extraction method; 154 specimens were extracted with both methods and subjected to PCR (polymerase chain reaction). The Chelex-100 procedure optimized the yield of DNA recovery and minimized contamination due to sample manipulation. It decreased false negative results due to PCR inhibitors. A DNA sample suitable for use in PCR was obtained in 30 minutes. Chelex-100 treatment is a simple, rapid and low-cost method for DNA extraction in clinical laboratories.

引用

页码：159 / 162

页数：4

共 12 条

[1]

A ONE-STEP MICROBIAL DNA EXTRACTION METHOD USING CHELEX-100 SUITABLE FOR GENE AMPLIFICATION [J].