DETERMINATION OF AFLATOXIN Q(1) IN URINE BY AUTOMATED IMMUNOAFFINITY COLUMN CLEANUP AND LIQUID-CHROMATOGRAPHY

被引：13

作者：

KUSSAK, A ^{[1
]}

ANDERSSON, B ^{[1
]}

ANDERSSON, K ^{[1
]}

机构：

[1] NATL INST OCCUPAT HLTH,DIV ANALYT CHEM,S-90713 UMEA,SWEDEN

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 656卷 / 02期

关键词：

D O I：

10.1016/0378-4347(94)00123-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A liquid chromatographic system with an automated clean-up procedure for aflatoxin Q(1) in human urine is described. The samples were cleaned up by using immunoaffinity columns originally designed for aflatoxin M(1). The chromatographic system was a C-18 column with an acidic mobile phase of acetonitrile-water containing potassium bromide. Fluorescence detection (365/440 nm) of aflatoxin Q(1) was enhanced by addition of bromine, using post-column derivatization, which was studied by factorial designs. Average recovery of aflatoxin Q(1) in spiked 10-ml urine samples was 88% (R.S.D. = 6.4%) at a level of 50 pg/ml. The determination limit was 49.5 pg/ml urine.

引用

页码：329 / 334

页数：6

共 18 条

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