Improvement of a solid-phase extraction method for determining biogenic amines in wines

Solid-phase extraction (SPE) was used simultaneously to clean up and concentrate samples prior to automatic derivatization to determine fifteen biogenic amines in wine. In the first step, SPE was used to remove polyphenolic compounds which interfere with further extraction and chromatographic analysis; in this study, treatments with polyvinylpyrrolidone, SAX and C-18 cartridges were tested and compared. In the second step, C-18 cartridges were used to concentrate the analytes after adding sodium octanesulfonate, sodium decanesulfonate and sodium dodecanesulfonate as ion-pair reagents. Reversed-phase chromatography with fluorimetric detection was performed on the extracted amines after automatic precolumn derivatization by treatment with o-phthalaldehyde, Biogenic amines can be separated and detected after the solid-phase extraction with an average sensitivity of the order of 20-90 mu g l(-1). Recoveries were determined by the standard addition technique and the overall method was successfully applied to the determination of the above-mentioned amines in red wines from the Tarragona region.