AUTOMATED DNA SEQUENCING AND ANALYSIS OF 106 KILOBASES FROM HUMAN CHROMOSOME-19Q13.3

被引：84

作者：

MARTINGALLARDO, A

MCCOMBIE, WR

GOCAYNE, JD

FITZGERALD, MG

WALLACE, S

LEE, BMB

LAMERDIN, J

TRAPP, S

KELLEY, JM

LIU, LI

DUBNICK, M

JOHNSTONDOW, LA

KERLAVAGE, AR

DEJONG, P

CARRANO, A

FIELDS, C

VENTER, JC

机构：

[1] NINCDS,RECEPTOR BIOCHEM & MOLEC BIOL SECT,BETHESDA,MD 20892

[2] LAWRENCE LIVERMORE NATL LAB,CTR HUMAN GENOME,LIVERMORE,CA 94551

[3] APPL BIOSYST INC,FOSTER CITY,CA 94404

来源：

NATURE GENETICS | 1992年 / 1卷 / 01期

关键词：

D O I：

10.1038/ng0492-34

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A total of 116,118 basepairs (bp) derived from three cosmids spanning the ERCC1 locus of human chromosome 19q13.3 have been sequenced with automated fluorescence-based sequencers and analysed by polymerase chain reaction amplification and computer methods. The assembled sequence forms two contigs totalling 105,831 bp, which contain a human fosB proto-oncogene, a gene encoding a protein phosphatase, two genes of unknown function and the previously-characterized ERCC1 DNA repair gene. This light band region has a high average density of 1.4 Alu repeats per kilobase. Human chromosome light bands could therefore contain up to 75,000 genes and 1.5 million Alu repeats.

引用

页码：34 / 39

页数：6

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