CHARACTERIZATION OF A HIGHLY POLYMORPHIC REGION NEAR THE 1ST EXON OF THE HUMAN MAOA GENE CONTAINING A GT DINUCLEOTIDE AND A NOVEL VNTR MOTIF

被引：62

作者：

HINDS, HL ^{[1
]}

HENDRIKS, RW ^{[1
]}

CRAIG, IW ^{[1
]}

CHEN, ZY ^{[1
]}

机构：

[1] UNIV OXFORD,DEPT BIOCHEM,GENET LAB,OXFORD OX1 3QU,ENGLAND

来源：

GENOMICS | 1992年 / 13卷 / 03期

关键词：

D O I：

10.1016/0888-7543(92)90181-Q

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The genes encoding the A and B forms of the human monoamine oxidase enzymes (MAOA and MAOB) are localized at Xp11.23-Xp11.4. We report the characterization of a highly informative polymorphic region within a 2.9-kb cloned fragment containing the first exon of the MAOA gene. The polymorphic region consists of a GT microsatellite directly adjacent to an imperfectly duplicated novel 23-bp VNTR motif. DNA sequencing within and flanking the repeated segment allowed the design of specific amplification primers. In 56 unrelated females, 15 different alleles were identified with sizes ranging from 285 to 388 bp. The alleles differed in both the number of dinucleotide and the number of VNTR repeats, yielding a highly informative polymorphic marker locus with a calculated heterozygosity value of 75%. © 1992.

引用

页码：896 / 897

页数：2

共 15 条

[11]

Sambrook J., 1989, MOL CLONING LAB MANU

[12] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].

SANGER, F ;

NICKLEN, S ;

COULSON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467

[13]

SIMS KB, 1989, AM J HUM GENET, V45, P424

[14] THE EMBL DATA LIBRARY [J].

STOEHR, PJ ;

CAMERON, GN .

NUCLEIC ACIDS RESEARCH, 1991, 19 :2227-2230

[15]

WEBER JL, 1990, NUCLEIC ACIDS RES, V18, P4635, DOI 10.1093/nar/18.15.4635-a

← 1 2 →