ENZYMATIC-PROPERTIES OF THE STONEFISH (SYNANCEIA-VERRUCOSA BLOCH AND SCHNEIDER, 1801) VENOM AND PURIFICATION OF A LETHAL, HYPOTENSIVE AND CYTOLYTIC FACTOR

被引：73

作者：

GARNIER, P ^{[1
]}

GOUDEYPERRIERE, F ^{[1
]}

BRETON, P ^{[1
]}

DEWULF, C ^{[1
]}

PETEK, F ^{[1
]}

PERRIERE, C ^{[1
]}

机构：

[1] UNIV PARIS SUD, FAC PHARM, BIOL & CONTROLE ORGANISMES PARASITES LAB, EQUIPE TOXINES & INSECTES, F-92290 CHATENAY MALABRY, FRANCE

来源：

TOXICON | 1995年 / 33卷 / 02期

关键词：

D O I：

10.1016/0041-0101(94)00151-W

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The crude venom of the stonefish (Synanceia verrucosa) possesses numerous enzymatic properties (hyaluronidase, eight esterases and ten aminopeptidases). Verrucotoxin was isolated by DEAE and hydroxyapatite chromatography, followed by FPLC gel filtration on Superdex 200 HR 10/30. It was found to be a glycoprotein with a mel. wt of 322,000 +/- 2000, comprising four subunits, 2 alpha (83,000) and 2 beta (78,000). Verrucotoxin, as the crude venom, is lethal for mice, haemolyses washed rabbit erythrocytes and induces a fall in arterial pressure in anaesthetized rats.

引用

页码：143 / 155

页数：13

共 58 条

[41] MARINE TOXINS AND VENOMOUS AND POISONOUS MARINE ANIMALS [J].