TRANSCRIPTIONAL REGULATION BY FOLATE - INDUCIBLE GENE-EXPRESSION IN DICTYOSTELIUM TRANSFORMANTS DURING GROWTH AND EARLY DEVELOPMENT

被引：62

作者：

BLUSCH, J ^{[1
]}

MORANDINI, P ^{[1
]}

NELLEN, W ^{[1
]}

机构：

[1] MAX PLANCK INST BIOCHEM,ZELLBIOL ABT,W-8033 MARTINSRIED,GERMANY

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 23期

关键词：

D O I：

10.1093/nar/20.23.6235

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Dictyostelium discoidin genes are induced in bacteria-grown cells shortly before the onset of development but are also highly expressed during growth in axenic medium. We here show that axenically growing cells strongly respond to the extracellular signal folate by suppressing discoidin synthesis while cell growth and development is not substantially affected. Repression occurs via two previously identified promoter elements, the dIE and the dAXE. Removal of the signal molecules or setting cells up for development results in rapid reactivation of the promoter. Based on this observation, we constructed the transformation vector pVEII and describe a convenient method which allows for controlled expression of a gene of interest in growing cells and also for external modulation in early development. Deletion constructs of the discoidin promoter can be used in addition to vary transcriptional activity over about one order of magnitude.

引用

页码：6235 / 6238

页数：4

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