THE 49-K SUBUNIT OF NADH - UBIQUINONE REDUCTASE (COMPLEX-I) FROM NEUROSPORA-CRASSA-MITOCHONDRIA - PRIMARY STRUCTURE OF THE GENE AND THE PROTEIN

被引：38

作者：

PREIS, D ^{[1
]}

VANDERPAS, JC ^{[1
]}

NEHLS, U ^{[1
]}

ROHLEN, DA ^{[1
]}

SACKMANN, U ^{[1
]}

JAHNKE, U ^{[1
]}

WEISS, H ^{[1
]}

机构：

[1] UNIV DUSSELDORF,INST ENZYMTECHNOL,W-5170 JULICH 1,GERMANY

来源：

CURRENT GENETICS | 1990年 / 18卷 / 01期

关键词：

NADH:ubiquinone reductase; Neurospora crassa; Primary structure;

D O I：

10.1007/BF00321116

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The primary structure of the 49 K subunit of the respiratory chain NADH:ubiquinone reductase (complex I) from Neurospora crassa was determined by sequencing cDNA, genomic DNA and the N-terminus of the mature protein. The sequence lengths correlate to a molecular mass of 54002 daltons for the preprotein and 49239 daltons for the mature protein. The presequence consists of 42 amino acids of typical composition for sequences which target nuclear-encoded proteins into mitochondria. The mature protein consists of 436 amino acids and shows 64% similarity to a 49 K subunit of bovine heart NADH:ubiquinone reductase and 33% to a predicted translation product of an open reading frame in the chloroplast DNAs of Marchantia polymorpha and Nicotiana tabacum. Evidence for an iron-sulfur cluster in the subunit is discussed. © 1990 Springer-Verlag.

引用

页码：59 / 64

页数：6

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