HOW MANY EF-TU MOLECULES PARTICIPATE IN AMINOACYL-TRANSFER RNA-BINDING

被引：24

作者：

BENSCH, K

PIEPER, U

OTT, G

SCHIRMER, N

SPRINZL, M

PINGOUD, A

机构：

[1] MED HSCH HANNOVER, ZENTRUM BIOCHEM, BIOPHYS CHEM ABT, KONSTANTY GUTSCHOW STR 8, W-3000 HANNOVER 61, GERMANY

[2] UNIV BAYREUTH, BIOCHEM LAB, W-8580 BAYREUTH, GERMANY

来源：

BIOCHIMIE | 1991年 / 73卷 / 7-8期

关键词：

ELONGATION FACTOR-TU; AMINOACYL-TRANSFER RNA; PROTEIN SYNTHESIS;

D O I：

10.1016/0300-9084(91)90146-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The stoichiometry of the EF-Tu.GTP.aminoacyl-tRNA complex has been re-determined by a variety of methods, viz gel filtrations, fluorescence titrations, as well as hydrolysis and RNase protection experiments. The results of these experiments clearly demonstrate that one aminoacyl-tRNA interacts with only one EF-Tu.GTP molecule, in agreement with the established view and in contrast to the recently published results by Ehrenberg et al [6].

引用

收藏

页码：1045 / 1050

页数：6

相关论文

共 46 条

[21] ROLE OF GUANOSINE 5'-TRIPHOSPHATE IN POLYPEPTIDE-CHAIN ELONGATION [J].

KAZIRO, Y .

BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 505 (01) :95-127

[22] EQUIVALENT AND NON-EQUIVALENT BINDING-SITES FOR TRANSFER-RNA ON AMINOACYL-TRANSFER-RNA SYNTHETASES [J].

KRAUSS, G ;

PINGOUD, A ;

BOEHME, D ;

RIESNER, D ;

PETERS, F ;

MAASS, G .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1975, 55 (03) :517-529

[23] A SIMPLIFIED PROCEDURE FOR THE ISOLATION OF BACTERIAL POLYPEPTIDE ELONGATION-FACTOR EF-TU [J].

LEBERMAN, R ;

ANTONSSON, B ;

GIOVANELLI, R ;

GUARIGUATA, R ;

SCHUMANN, R ;

WITTINGHOFER, A .

ANALYTICAL BIOCHEMISTRY, 1980, 104 (01) :29-36

[24] KINETIC-STUDIES OF ESCHERICHIA-COLI ELONGATION FACTOR-TU-GUANOSINE 5'-TRIPHOSPHATE-AMINOACYL-TRANSFER RNA COMPLEXES [J].

LOUIE, A ;

JURNAK, F .

BIOCHEMISTRY, 1985, 24 (23) :6433-6439

[25]

LOUIE A, 1984, J BIOL CHEM, V259, P5010

[26]

Miller D., 1977, MOL MECH PROTEIN BIO, P323

[27]

Miller D L, 1974, Methods Enzymol, V30, P219

[28] INTERACTION OF GUANOSINE 5'-DIPHOSPHATE, 2' (3')-DIPHOSPHATE WITH BACTERIAL ELONGATION FACTOR TU [J].

MILLER, DL ;

CASHEL, M ;

WEISSBACH, H .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1973, 154 (02) :675-682

[29]

OSTERBERG R, 1981, EUR J BIOCHEM, V117, P155

[30] THE INFLUENCE OF DIFFERENT MODIFICATIONS OF ELONGATION FACTOR-TU FROM ESCHERICHIA-COLI ON TERNARY COMPLEX-FORMATION INVESTIGATED BY FLUORESCENCE SPECTROSCOPY [J].

OTT, G ;

JONAK, J ;

ABRAHAMS, IP ;

SPRINZL, M .

NUCLEIC ACIDS RESEARCH, 1990, 18 (03) :437-441

← 1 2 3 4 5 →